Timothy C Dodge

US Patent:

7033804, Apr 25, 2006

Filed:

Apr 4, 2002

Appl. No.:

10/116821

Inventors:

Timothy C. Dodge - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12P 7/60
C12P 7/58

US Classification:

435138, 435137

Abstract:

The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.

US Patent:

7241587, Jul 10, 2007

Filed:

Apr 4, 2002

Appl. No.:

10/117283

Inventors:

Timothy C. Dodge - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12P 1/00
C12P 19/28
C12N 9/10
C12N 1/20
C07H 21/04

US Classification:

435 41, 435 85, 435 89, 435105, 435183, 435193, 4352523, 536 232

Abstract:

The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.

US Patent:

7407780, Aug 5, 2008

Filed:

Nov 13, 2006

Appl. No.:

11/598375

Inventors:

Timothy C. Dodge - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

Assignee:

Genencor International, Inc. - Palo Alto CA

International Classification:

C12P 17/04
C12N 9/12
C12N 1/20
C12N 15/74
C07H 21/04

US Classification:

435126, 435183, 435194, 4352523, 435471, 536 232

Abstract:

The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.

US Patent:

7419795, Sep 2, 2008

Filed:

May 10, 2004

Appl. No.:

10/842929

Inventors:

Timothy C. Dodge - Sunnyvale CA, US
Manoj Kumar - Fremont CA, US
M. Harunur Rashid - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

Assignee:

Danisco A/S, Genencor Division - Palo Alto CA

International Classification:

G01N 33/53

US Classification:

435 76, 435 71, 435137, 514 2, 514 12

Abstract:

The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.

US Patent:

8034583, Oct 11, 2011

Filed:

Jun 18, 2008

Appl. No.:

12/141522

Inventors:

Timothy C. Dodge - Sunnyvale CA, US
Manoj Kumar - Fremont CA, US
M. Harunur Rashid - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

Assignee:

Danisco US Inc. - Palo Alto CA

International Classification:

C12P 21/06

US Classification:

435 691, 435320, 4352523, 435471, 435 71, 536 237, 536 231, 530350, 530300

Abstract:

The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.

US Patent:

20050106693, May 19, 2005

Filed:

Jul 27, 2004

Appl. No.:

10/899602

Inventors:

Timothy Dodge - Sunnyvale CA, US
M. Rashid - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

International Classification:

C12P007/58
C12P007/60
C12N015/74
C12N001/21

US Classification:

435138000, 435252300, 435252330, 435252310, 435488000

Abstract:

The present invention relates to a method of altering bacterial host cells to accumulate 2-keto-D-gluconic acid (2-KDG) by inactivating an endogenous membrane bound 2-keto-D-gluconate dehydrogenase (2-KDGDH), which prior to inactivation catalyzed the conversion of 2-KDG to 2,5-diketogluconate (2,5-DKG).

US Patent:

20060068477, Mar 30, 2006

Filed:

Nov 17, 2005

Appl. No.:

11/282000

Inventors:

Timothy Dodge - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

International Classification:

C12P 17/04
C12P 7/60

US Classification:

435126000, 435138000

Abstract:

The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.

US Patent:

20070184540, Aug 9, 2007

Filed:

Apr 2, 2007

Appl. No.:

11/731923

Inventors:

Timothy Dodge - Sunnyvale CA, US
M. Rashid - Sunnyvale CA, US
Fernando Valle - Burlingame CA, US

International Classification:

C12P 7/60
C12N 9/02
C12N 1/21
C12N 15/74

US Classification:

435138000, 435252300, 435252330, 435189000, 435471000

Abstract:

The present invention relates to a method of altering bacterial host cells to accumulate 2-keto-D-gluconic acid (2-KDG) by inactivating an endogenous membrane bound 2-keto-D-gluconate dehydrogenase (2-KDGDH), which prior to inactivation catalyzed the conversion of 2-KDG to 2,5-diketogluconate (2,5-DKG).