Timothy J Olsen

US Patent:

20220403465, Dec 22, 2022

Filed:

Nov 13, 2020

Appl. No.:

17/776655

Inventors:

- New York NY, US
Timothy R. OLSEN - New York NY, US

International Classification:

C12Q 1/6874
C12N 15/10

Abstract:

Single-cell multi-omics by co-encapsulating a single cell with two beads, the first an RNA barcoding bead having barcoded mRNA capture primer oligonucleotides attached on the bead surface; and the second a DNA barcoding bead having two types of oligonucleotides releasably attached to the surface: (1) barcoded adapter oligonucleotides that are complementary to oligonucleotides bound to the transposase that are eventually incorporated into gDNA fragments and (2) polyadenylated barcoded oligonucleotides containing the same barcode sequence as the adapters. In addition, integrated analysis of RNA and protein, including intracellular protein, from individual cells using similar co-encapsulation of a single cell, an RNA barcoding bead, and with/without a specific or non-specific protein binding bead in a microwell, to avoid protein fixation by first lysing the cell to liberate intracellular contents, and then capturing protein either on a solid surface or in solution with barcoded affinity reagents.

US Patent:

20170067091, Mar 9, 2017

Filed:

Sep 19, 2016

Appl. No.:

15/269494

Inventors:

- New York NY, US
Jing Zhu - Beijing, CN
Timothy Olsen - New York NY, US

International Classification:

C12Q 1/68
B01L 7/00
B01L 3/00

Abstract:

A microdevice for isolating and amplifying aptamers includes a selection microchamber and an amplification microchamber. The selection microchamber can include a plurality of cultured cells immobilized therein. A first microchannel connecting the selection microchamber to the amplification microchamber can be configured to hydrodynamically transfer oligomers from the selection microchamber to the amplification chamber. A second microchannel connecting the selection microchamber to the amplification microchamber can be configured to hydrodynamically transfer oligomers from the amplification chamber to the selection chamber.