Timothy E Pelletier

US Patent:

20210002720, Jan 7, 2021

Filed:

Aug 21, 2020

Appl. No.:

16/999329

Inventors:

- Germantown MD, US
Jimmy Adediran - Waltham MA, US
Timothy Pelletier - Leominster MA, US
Austin Ricker - Waltham MA, US
Angela DeLucia - Cambridge MA, US
Ben McNally - Medford MA, US
Dona Hevroni - Lexington MA, US
Mong Sano Marma - Natick MA, US
John Andrew Sheridan - Marblehead MA, US

International Classification:

C12Q 1/6869
C12Q 1/6876
C12Q 1/6806

Abstract:

The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3′-O position capped by a disulfide-based 3′-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

US Patent:

20190226018, Jul 25, 2019

Filed:

Jan 24, 2018

Appl. No.:

15/878633

Inventors:

- Germantown MD, US
Jimmy Adediran - Waltham MA, US
Timothy Pelletier - Leominster MA, US
Austin Ricker - Waltham MA, US
Angela DeLucia - Cambridge MA, US
Ben McNally - Medford MA, US
Dona Hevroni - Lexington MA, US
Mong Sano Marma - Natick MA, US
John Andrew Sheridan - Marblehead MA, US

International Classification:

C12Q 1/6869
C12Q 1/6876
C12Q 1/6806

Abstract:

The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3′-O position capped by a disulfide-based 3′-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

US Patent:

20180127809, May 10, 2018

Filed:

Oct 31, 2017

Appl. No.:

15/799139

Inventors:

- Waltham MA, US
Michael Georges Perbost - Belmont MA, US
Dona Hevroni - Lexington MA, US
Minakshi Gupta - Wakefield MA, US
Austin Ricker - Waltham MA, US
Timothy Pelletier - Fitchburg MA, US

International Classification:

C12Q 1/6806
C07C 215/28
C07D 311/66
C12Q 1/6874
G01N 33/58

Abstract:

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of photoprotective mixture of compounds as imaging reagents to improve stability and storage of fluorescent compounds, including but not limited to, nucleotides with fluorescent labels.