Todd M Rearick

US Patent:

8263336, Sep 11, 2012

Filed:

May 31, 2011

Appl. No.:

13/149331

Inventors:

Jonathan M Rothberg - Guilford CT, US
James M. Bustillo - Castro Valley CA, US
Mark J. Milgrew - Branford CT, US
Jonathan C. Schultz - Oxford MA, US
David Marran - Durham CT, US
Todd M Rearick - Cheshire CT, US
Kim L. Johnson - Carlsbad CA, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12M 1/00
C12M 1/34
C12M 3/00
G01N 27/403

US Classification:

435 61, 4352831, 4352872, 257253

Abstract:

Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

US Patent:

8552771, Oct 8, 2013

Filed:

Apr 22, 2013

Appl. No.:

13/867959

Inventors:

Todd Rearick - Cheshire CT, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

H03L 7/00

US Classification:

327141

Abstract:

A method including receiving an output signal from a sensor array, providing power to the sensor array and to the first analog-to-digital converter, generating a plurality of clock signals that are synchronous with a primary clock signal, providing a first clock signal of the plurality to the first analog-to-digital converter and providing a second clock signal of the plurality to the first switcher. The first clock signal is asynchronous with the second clock signal.

US Patent:

8592153, Nov 26, 2013

Filed:

Sep 5, 2012

Appl. No.:

13/603631

Inventors:

James Bustillo - Castro Valley CA, US
Jonathan Schultz - Oxford MA, US
Todd Rearick - Cheshire CT, US
Mark Milgrew - Branford CT, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12M 1/34
C12M 3/00
G01N 27/403

US Classification:

435 61, 4352871, 4352872, 257253

Abstract:

Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

US Patent:

8592154, Nov 26, 2013

Filed:

Sep 7, 2012

Appl. No.:

13/607433

Inventors:

Todd Rearick - Cheshire CT, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12M 1/34
C12M 3/00
G01N 27/403

US Classification:

435 61, 4352871, 4352872, 257253

Abstract:

Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

US Patent:

20100300895, Dec 2, 2010

Filed:

May 24, 2010

Appl. No.:

12/785716

Inventors:

John Nobile - Guilford CT, US
Thomas Roth - Guilford CT, US
Todd Rearick - Cheshire CT, US
Jonathan Schultz - Oxford MA, US
Jonathan Rothberg - Guilford CT, US
David Marran - Durham CT, US

Assignee:

ION TORRENT SYSTEMS, INC. - South San Francisco CA

International Classification:

G01N 27/26

US Classification:

205775, 204435

Abstract:

The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors underminimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.

US Patent:

20100301398, Dec 2, 2010

Filed:

May 29, 2009

Appl. No.:

12/475311

Inventors:

Jonathan M. Rothberg - Guilford CT, US
James M. Bustillo - Castro Valley CA, US
Mark J. Milgrew - Branford CT, US
Jonathan C. Schultz - Oxford MA, US
David Marran - Durham CT, US
Todd M. Rearick - Cheshire CT, US
Kim L. Johnson - Carlsbad CA, US

Assignee:

Ion Torrent Systems Incorporated - Guilford CT

International Classification:

H01L 29/78
H01L 21/336

US Classification:

257253, 438 49, 257E29255, 257E21409

Abstract:

Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

US Patent:

20110263463, Oct 27, 2011

Filed:

Oct 22, 2009

Appl. No.:

13/125133

Inventors:

Jonathan M. Rothberg - Guilford CT, US
James Bustillo - Castro Valley CA, US
Mark Milgrew - Branford CT, US
Jonathan Schultz - Oxford MA, US
David Marran - Durham CT, US
Todd Rearick - Cheshire CT, US
Kim Johnson - Carlsbad CA, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C40B 60/12
B82Y 15/00

US Classification:

506 39, 977755, 977957, 977958, 977811

Abstract:

The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

US Patent:

20110318820, Dec 29, 2011

Filed:

Jun 29, 2011

Appl. No.:

13/172048

Inventors:

Wolfgang Hinz - Killingworth CT, US
David Light - New Haven CT, US
Todd Rearick - Cheshire CT, US
James Ball - Ledyard CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12M 1/34

US Classification:

4352872

Abstract:

The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.