Tricia A Patterson

US Patent:

20060065531, Mar 30, 2006

Filed:

Sep 22, 2005

Appl. No.:

11/234054

Inventors:

Daniel Smolko - Jamul CA, US
Paul Swanson - Santee CA, US
Dalibor Hodko - Poway CA, US
David Canter - San Diego CA, US
Robert Haigis - San Diego CA, US
Tricia Patterson - San Diego CA, US

Assignee:

Nanogen, Inc - San Diego CA

International Classification:

C07K 1/26
G01N 27/447

US Classification:

204450000, 204600000

Abstract:

Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.