Vaibhav Pandit

US Patent:

20160230189, Aug 11, 2016

Filed:

Sep 23, 2014

Appl. No.:

15/024264

Inventors:

- Troy NY, US
Andre Ronald WATSON - Troy NY, US
Vaibhav A. PANDIT - Troy NY, US

Assignee:

RENSSELAER POLYTECHNIC INSTITUTE - Troy NY

International Classification:

C12N 15/85

Abstract:

An improved nanoparticle for transfecting cells is provided. The nanoparticle includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating , where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide. Also provided is an improved method of modifying intracellular polynucleotides. The method includes contacting a cell with a nanoparticle that includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating, where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide.