Valerie A Villareal

US Patent:

20210389245, Dec 16, 2021

Filed:

Oct 4, 2019

Appl. No.:

17/282807

Inventors:

- Natick MA, US
Alan Lloyd Weeks - S. Easton MA, US
Valerie Villareal - Boston MA, US
Patrick Murphy - Allston MA, US
Eric Anthony Robertson - San Antonio TX, US
Deirdre Ellen Day - Winchester MA, US
Matthew Bernard Dean - Far Hills NJ, US
Todd Glendon Campbell - Holliston MA, US
Brian Christopher Burke - Mahwah NJ, US
Tyler S. Smith - Cambridge MA, US
Thomas Christopher Hartner - Pepperell MA, US
Stanley Owen Thompson - New Boston NH, US
Nhat Nam Trinh - Quincy MA, US
David Carpenter - Jaffrey NH, US
Gregory J. Kintz - Santa Cruz CA, US
Paul Koh - New York NY, US
David Jennings Dostal - Hanover NH, US
Kevin Doherty - Palo Alto CA, US
Joel F. Jensen - Redwood City CA, US
William Law - Palo Alto CA, US
J. Efraín Alcorta - Austin TX, US

International Classification:

G01N 21/64
B01L 3/00

Abstract:

The present disclosure is drawn to devices and systems for target detection in samples, particularly allergen detection in food samples. The allergen detection system includes a sampler, a disposable analysis cartridge and a detection device with an optimized optical system. The allergen detection utilizes nucleic acid molecules as detection agents and detection probes.

US Patent:

20210311032, Oct 7, 2021

Filed:

Feb 21, 2019

Appl. No.:

16/971701

Inventors:

- Natick MA, US
Alan Lloyd Weeks - S. Easton MA, US
Valerie Villareal - Boston MA, US
Patrick Murphy - Allston MA, US
Eric Anthony Robertson - San Antonio TX, US
David Carpenter - Jaffrey NH, US
Deirdre Ellen Day - Winchester MA, US
Matthew Bernard Dean - Far Hills NJ, US
Todd Glendon Campbell - Holliston MA, US
Gregory J. Kintz - Santa Cruz CA, US
Paul Koh - New York NY, US
David Jennings Dostal - Hanover NH, US
Kevin Doherty - San Francisco CA, US
Joel F. Jensen - Redwood City CA, US
William Law - Palo Alto CA, US
J. Efraín Alcorta - Austin TX, US

International Classification:

G01N 33/53
G01N 33/543
C12Q 1/6816
G01N 33/02

Abstract:

The present invention is drawn to devices and systems for allergen detection in food samples. The allergen detection system includes a disposable analysis cartridge and a detection device with an optimized optical system.

US Patent:

20210109076, Apr 15, 2021

Filed:

Dec 21, 2020

Appl. No.:

17/128270

Inventors:

- Natick MA, US
Renuka Babu Brown - Weston MA, US
Sarah Stidham - Brighton MA, US
Valerie Villareal - Boston MA, US
Adam J. Young - Dedham MA, US
Joshua Glenn Anthony - Reading MA, US
Patrick Murphy - Allston MA, US
John H. Kepler - Lexington MA, US
Adam Jacobs - Hollis NH, US

International Classification:

G01N 33/02
C12M 1/34
G01N 1/08
C12N 15/115
G01N 1/14
G01N 1/28
G01N 33/53
G01N 33/58

Abstract:

The present invention provides systems, devices, signaling polynucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluorescent signal. SPNs derived from aptamer that bind allergens are provided as detection agents. SPNs have a single open structure, and are labeled with a fluorophore. Changes in fluorescence polarization of SPNs upon the binding of allergens are measured to calculate the allergen content in a sample.

US Patent:

20190292544, Sep 26, 2019

Filed:

Nov 8, 2017

Appl. No.:

16/347935

Inventors:

- Natick MA, US
Valerie Villareal - Boston MA, US
Sarah Stidham - Brighton MA, US
Morris Nehama - Needham MA, US

International Classification:

C12N 15/115
G01N 33/02
C12N 15/10
G01N 33/533

Abstract:

The present application discloses nucleic acid aptamer based signaling polynucleotides (SPNs) that specifically bind an allergen protein. Provided in the present invention include aptamers, SPNs, SPN-complement complexes, magnetic particle conjugates, DNA printed glass slides and detection agents, and detection methods using the same for detecting the presence, or absence of an allergen protein in a food sample.

US Patent:

20190079063, Mar 14, 2019

Filed:

Mar 10, 2017

Appl. No.:

16/084996

Inventors:

- Natick MA, US
Renuka Babu Brown - Weston MA, US
Sarah Stidham - Brookline MA, US
Valerie Villareal - Boston MA, US
Adam J. Young - Dedham MA, US
Joshua Glenn Anthony - Reading MA, US
Patrick Murphy - Cambridge MA, US
John H. Kepler - Lexington MA, US
Adam Jacobs - Hollis NH, US

International Classification:

G01N 33/02
G01N 33/53
G01N 33/58
G01N 1/28

Abstract:

The present invention provides systems, devices, signaling polynucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluorescent signal. SPNs derived from aptamer that bind allergens are provided as detection agents. SPNs have a single open structure, and are labeled with a fluorophore. Changes in fluorescence polarization of SPNs upon the binding of allergens are measured to calculate the allergen content in a sample.