Skills:
Laboratory: PCR, gel electrophoresis and imaging for DNA and RNA analysis, bacterial cell culture and plasmid preparation, site-directed mutagenesis, restriction digests, ligations, standard cloning, Gateway cloning, RNA/DNA extraction from tissue, immunohistochemistry, Western blotting, reverse transcription PCR, phenol/chloroform purification, in vitro RNA transcription, ELISA, confocal and fluorescence microscopy. Computer: Microsoft Office Specialist (MOS) Certification in Word, Excel, and PowerPoint (May 2005), DNA sequence analysis using MacVector and ApE, PCR primer design using Amplify, LIMS. Zebrafish: Experienced in husbandry, mating, staging, manual and chemical dechorionation of embryos, microinjection of DNA and RNA into embryos, fin-clipping and genomic DNA extraction for genotyping, in vitro fertilization techniques.