Vincent P Martin

US Patent:

7172886, Feb 6, 2007

Filed:

Dec 6, 2001

Appl. No.:

10/006909

Inventors:

Jay Keasling - Berkeley CA, US
Vincent Martin - Kensington CA, US
Douglas Pitera - Berkeley CA, US
Seon-Won Kim - Jeongdong-myeon Sacheon, KR
Yasuo Yoshikuni - Berkeley CA, US
Jack Newman - San Francisco CA, US
Artem Valentinovich Khlebnikov - Mountain View CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12P 7/00
C07H 21/04
C12N 9/00
C12N 1/20
C12N 15/00

US Classification:

435132, 435183, 435189, 435194, 435232, 4352523, 4353201, 536 232

Abstract:

Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

US Patent:

7192751, Mar 20, 2007

Filed:

Apr 9, 2003

Appl. No.:

10/411066

Inventors:

Jay Keasling - Berkeley CA, US
Vincent Martin - Kensington CA, US
Douglas Pitera - Oakland CA, US
Jack Newman - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12P 7/42
C07H 21/04
C12N 9/00
C12N 1/20
C12N 15/00

US Classification:

435146, 435 41, 435183, 435194, 435232, 4352523, 4353201, 536 232

Abstract:

Methods for synthesizing amorpha-4,11-diene synthase from isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. Amorpha-4,11-diene synthase is then produced using an optimized amorpha-4,11-diene synthase gene. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

US Patent:

20070141574, Jun 21, 2007

Filed:

Sep 29, 2004

Appl. No.:

10/573796

Inventors:

Jay Keasling - Berkely CA, US
Jack Newman - Berkeley CA, US
Douglas Pitera - Oakland CA, US
Sydnor Withers - Richmond CA, US
Keith Reiling - San Francisco CA, US
Vincent Martin - Kensington CA, US

International Classification:

C12Q 1/68
C12N 15/74

US Classification:

435006000, 435455000, 435471000

Abstract:

The present invention provides isolated, genetically modified host cells, where a host cell is genetically modified with a nucleic acid that includes a nucleotide sequence encoding a biosynthetic pathway enzyme. Synthesis of the enzyme in the host cell results in conversion of a substrate for the enzyme into a biosynthetic pathway intermediate, which intermediate is produced in an amount effective to inhibit growth of the genetically modified host cell. The present invention further provides compositions and kits comprising a subject genetically modified host cell. Subject host cells are useful for identifying a gene product having activity in a biosynthetic pathway. The present invention further provides methods of identifying a gene product having activity in a biosynthetic pathway.

US Patent:

20070166782, Jul 19, 2007

Filed:

Sep 1, 2006

Appl. No.:

11/469587

Inventors:

JAY KEASLING - Berkeley CA, US
VINCENT MARTIN - Kensington CA, US
DOUGLAS PITERA - Oakland CA, US
SEON-WON KIM - Jeongdong-Myeon, Sacheon City, KR
SYDNOR WITHERS - Richmond CA, US
YASUO YOSHIKUNI - Berkeley CA, US
JACK NEWMAN - Berkeley CA, US
ARTEM KHLEBNIKOV - Mountain View CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - San Francisco CA

International Classification:

C12P 33/00
C12P 17/00
C12P 9/00
C12N 9/12
C07H 21/04
C12N 15/74
C12N 1/21

US Classification:

435052000, 435117000, 435131000, 435252300, 435471000, 435194000, 536023200

Abstract:

Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

US Patent:

20070264632, Nov 15, 2007

Filed:

Dec 8, 2004

Appl. No.:

10/581975

Inventors:

Jack Newman - Berkeley CA, US
Neil Renninger - Albany CA, US
Vincent Martin - Kensington CA, US
Jay Keasling - Berkeley CA, US
Keith Reiling - Oakland CA, US

International Classification:

G01N 33/573
C12Q 1/68

US Classification:

435006000, 435007400

Abstract:

The present invention provides a method of identifying an enzyme, the method generally involving contacting a sample containing an enzyme with a selected enzyme substrate, where the contacting provides for covalent binding of the substrate to an amino acid of the enzyme to form a covalently modified enzyme; and determining the amino acid sequence of at least a portion of the covalently modified enzyme, using any available peptide sequencing technology, such as tandem mass spectrometry. The present invention further provides methods of identifying a nucleic acid encoding an enzyme, the methods generally involving identifying an enzyme; and, based on the amino acid sequence of at least a portion of the enzyme, designing nucleic acid probes or primers that hybridize to the nucleic acid encoding the enzyme.