Virginia M Crews

US Patent:

20090123910, May 14, 2009

Filed:

Nov 14, 2007

Appl. No.:

11/985547

Inventors:

Adrien P. Malick - Granite MD, US
Virginia M. Crews - Baltimore MD, US
Julie L. Rosales - Randallstown MD, US
Carrie S. Ferguson - Bel Air MD, US
Jeff H. Bruton - Randallstown MD, US
Robert J. Beadenkopf - Pasadena MD, US

International Classification:

C12Q 1/70
C12P 21/00
G01N 33/50
C12Q 1/00
G01N 33/567

US Classification:

435 5, 435 701, 435 703, 435 405, 435 4, 435 792, 435 721

Abstract:

Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

US Patent:

20070292899, Dec 20, 2007

Filed:

May 11, 2007

Appl. No.:

11/747830

Inventors:

Stephen Lovell - Sparks MD, US
Lydia Blank - Sparks MD, US
Virginia Crews - Sparks MD, US
Nancy Hasse - Sparks MD, US
Peter Lu - Sunnyvale CA, US
Johannes Schweizer - Sunnyvale CA, US

International Classification:

C12P 21/04
G01N 33/00
G01N 33/53

US Classification:

435007210, 435007920, 435070300

Abstract:

Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.

US Patent:

20150266928, Sep 24, 2015

Filed:

Jan 15, 2015

Appl. No.:

14/598148

Inventors:

- Sunnyvale CA, US
- Franklin Lakes NJ, US
Virginia Crews - Sparks MD, US
Nancy Hasse - Sparks MD, US
Peter Lu - Sunnyvale CA, US
Johannes Schweizer - Sunnyvale CA, US
John Mantlo - Westminster MD, US

International Classification:

C07K 14/005
C07K 14/82
C12N 7/00
C07K 1/14

Abstract:

Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.