Vivian L Mackay

US Patent:

46135723, Sep 23, 1986

Filed:

Aug 15, 1983

Appl. No.:

6/523652

Inventors:

Vivian L. MacKay - Seattle WA
Thomas R. Manney - Manhattan KS

Assignee:

Kansas State University Research Foundation - Manhattan KS
Rutgers Research and Educational Foundation - Piscataway NJ

International Classification:

C12N 120
C12N 118
C12N 1500
C12N 116

US Classification:

435253

Abstract:

The yeast BAR1 structural gene is disclosed. The gene was cloned in E. coli and expressed in two different yeasts, Saccharomyces and Schizosaccharomyces.

US Patent:

56482191, Jul 15, 1997

Filed:

Jun 7, 1995

Appl. No.:

8/479882

Inventors:

Vivian L. MacKay - Seattle WA
Emma E. Moore - Seattle WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12Q 168
C12Q 102
C12N 502
C12N 1512

US Classification:

435 6

Abstract:

The present invention provides an immature dendritic cell line derived from p53 growth suppressor gene deficient animals. The immature dendritic cell line may be induced to become an activated dendritic cell line that will stimulate T-cells to proliferate. The cell line is useful for presentation of antigens involved in autoimmune disease and analysis of peptides that produce a T-cell response.

US Patent:

50377434, Aug 6, 1991

Filed:

Nov 14, 1988

Appl. No.:

7/270933

Inventors:

Susan K. Welch - Los Altos Hills CA
Vivian L. MacKay - Seattle WA
Carli L. Yip - Bellevue WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12N 116
C12N 506
C12N 1511
C12N 1579

US Classification:

435 691

Abstract:

Methods for producing heterologous proteins in a host organism whereby the proteins are processed through the secretory pathway of the host are provided. Secretion is achieved by transforming a host organism with a DNA construct comprising a transcriptional promoter operably linked to DNA sequences encoding a signal peptide, at least a portion of the BAR1 C-terminal domain capable of directing the export of heterologous proteins and a heterologous protein or polypeptide. DNA constructs and transformants are also provided wherein the DNA sequence encoding at least a portion of the C-terminal domain of BAR1 capable of directing the export of heterologous proteins further comprises a DNA sequence encoding a proteolytic cleavage site operably linked to the DNA sequence encoding a heterologous protein.

US Patent:

51358549, Aug 4, 1992

Filed:

May 3, 1988

Appl. No.:

7/189547

Inventors:

Vivian L. MacKay - Seattle WA
Susan K. Welch - Seattle WA
Carli L. Yip - Bellevue WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12N 1500
C12N 1581
C12N 119
C12P 2102

US Classification:

435 691

Abstract:

Methods for producing a heterologous protein or polypeptide are disclosed. A preferred method utilizes a fungal cell carrying a defect in a gene whose product is required for the addition of outer chain oligosaccharide moieties to glycoproteins, the cell transformed with a first DNA construct comprising a regulated promoter followed downstream by a DNA sequence which complements the defect, and a second DNA construct comprising a second promoter followed downstream by a DNA sequence encoding a secretion signal and a DNA sequence encoding a heterologous protein or polypeptide. A yeast cell having a Mnn9. sup. - phenotype and capable of producing colonies of normal morphologies in the absence of osmotic stabilization is also disclosed.