Vivian Xiao from San Lorenzo, CA

Methods Employing Generalized Target-Binding E-Tag Probes
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US Patent:

6818399, Nov 16, 2004
Filed:

Apr 2, 2001
Appl. No.:

09/824984
Inventors:

Sharat Singh - San Jose CA
Vivian Xiao - San Jose CA
Assignee:

Aclara Biosciences, Inc. - Mountain View CA
International Classification:

C12O 168
US Classification:

435 6, 536 242, 536 2431, 536 2433, 536 231, 536 2532, 536 266
Abstract:

Methods for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The methods include the use of electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In practicing the methods, target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

Generalized Target-Binding E-Tag Probe Compositions
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US Patent:

20020058263, May 16, 2002
Filed:

Apr 2, 2001
Appl. No.:

09/824861
Inventors:

Sharat Singh - San Jose CA, US
Vivian Xiao - Cupertino CA, US
International Classification:

C12Q001/68
G01N033/53
US Classification:

435/006000, 435/007500
Abstract:

Compositions for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. The compositions include one or more uncleaved or partially cleaved electrophoretic tag (e-tag) probes from a set of e-tag probes, at least one e-tag reporter out of a possible set of e-tag reporters and a capture agent. Detection involves the release of identifying tags as a consequence of target recognition. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.

Sample Evaporative Control
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US Patent:

20020182749, Dec 5, 2002
Filed:

Nov 2, 2001
Appl. No.:

10/002883
Inventors:

Sharat Singh - San Jose CA, US
Vivian Xiao - San Jose CA, US
Ian Gibbons - Portola Valley CA, US
Travis Boone - Oakland CA, US
Torleif Bjornson - Gilroy CA, US
Herbert Hooper - Belmont CA, US
Edwin Ullman - Atherton CA, US
Assignee:

ACLARA BioSciences, Inc.
International Classification:

G01N033/558
US Classification:

436/514000
Abstract:

Devices and methods are provided using microfluidic devices for manipulating small volumes and determining a variety of chemical and physical events. The devices rely upon an opening to the atmosphere of a small volume in a zone, where a sample is placed in the zone where evaporation can occur. The zone is maintained in contact with a liquid medium that serves to replenish the liquid in the zone and maintain the composition of the mixture in the zone substantially constant. The diffusion of components in the zone is restricted during the course of the determination by the liquid flux into the zone.

Sample Evaporative Control
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US Patent:

20030068646, Apr 10, 2003
Filed:

Sep 27, 2001
Appl. No.:

09/965448
Inventors:

Sharat Singh - San Jose CA, US
Vivian Xiao - San Jose CA, US
Ian Gibbons - Portola Valley CA, US
Travis Boone - Oakland CA, US
Torleif Bjornson - Gilroy CA, US
Herbert Hooper - Belmont CA, US
Edwin Ullman - Atherton CA, US
International Classification:

G01N033/53
US Classification:

435/007100
Abstract:

Devices and methods are provided using microfluidic devices for manipulating small volumes and determining a variety of chemical and physical events. The devices rely upon an opening to the atmosphere of a small volume in a zone, where a sample is placed in the zone where evaporation can occur. The zone is maintained in contact with a liquid medium that serves to replenish the liquid in the zone and maintain the composition of the mixture in the zone substantially constant. The diffusion of components in the zone is restricted during the course of the determination by the liquid flux into the zone.

Detection Of Nucleic Acid Sequences By Cleavage And Separation Of Tag-Containing Structures
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US Patent:

20040067498, Apr 8, 2004
Filed:

Nov 6, 2002
Appl. No.:

10/289309
Inventors:

Ahmed Chenna - Sunnyvale CA, US
Vivian Xiao - Cupertino CA, US
Sharat Singh - San Jose CA, US
International Classification:

C12Q001/68
US Classification:

435/006000
Abstract:

The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.

Detection Of Nucleic Acid Sequences By Cleavage And Separation Of Tag-Containing Structures
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US Patent:

20060223107, Oct 5, 2006
Filed:

Jun 5, 2006
Appl. No.:

11/447753
Inventors:

Ahmed Chenna - Sunnyvale CA, US
Vivian Xiao - Cupertino CA, US
Sharat Singh - San Jose CA, US
International Classification:

C12Q 1/68
C07H 21/04
US Classification:

435006000, 536024100
Abstract:

The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.

Sample Evaporative Control
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US Patent:

6627406, Sep 30, 2003
Filed:

May 10, 2000
Appl. No.:

09/568786
Inventors:

Sharat Singh - San Jose CA
Vivian Xiao - San Jose CA
Ian Gibbons - Portola Valley CA
Travis Boone - Oakland CA
Torleif Ove Bjornson - Gilroy CA
Herbert H. Hooper - Belmont CA
Edwin F. Ullman - Atherton CA
Assignee:

Aclara BioSciences, Inc. - Mountain View CA
International Classification:

G01N 3353
US Classification:

435 71, 435 8, 422 50, 422 58, 422 99
Abstract:

Devices and methods are provided using microfluidic devices for manipulating small volumes and determining a variety of chemical and physical events. The devices rely upon an opening to the atmosphere of a small volume in a zone, where a sample is placed in the zone where evaporation can occur. The zone is maintained in contact with a liquid medium that serves to replenish the liquid in the zone and maintain the composition of the mixture in the zone substantially constant. The diffusion of components in the zone is restricted during the course of the determination by the liquid flux into the zone.