Vrushalee V Palsule

Tampa And St Petersburg, Florida Area

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Location:

12517 Streamdale Dr, Tampa, FL 33626

Industry:

Hospital & Health Care

Work:

University of Toledo since Oct 2010
Research Technician
Louisiana Tech University Aug 2008 - May 2010
Research assistant

Education:

Louisiana Tech University 2008 - 2009
Master's degree, Cell/Cellular and Molecular Biology KC college, Mumbai 2004 - 2007
Bachelor of Science (BS), Biotechnology

Skills:

Polymerase Chain Reaction
Dna Extraction
Microsoft Excel
Microsoft Office
Real Time Polymerase Chain Reaction
Data Analysis
Molecular Biology
Research
Google Docs
Microsoft Word
Microsoft Powerpoint
Communication
Presentation Skills
Process Quality Improvement

Languages:

English

Vrushalee Palsule Tampa, FL

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Work:

Lake Erie Research Center
Toledo, OH
Oct 2010 to Jul 2013
Research Technician Louisiana Tech University
Ruston, LA
2009 to 2010
Teaching Assistant Louisiana Tech University
Ruston, LA
2008 to 2009
Research Assistant Thyrocare, Thyroid Hormones Testing Laboratory
Mumbai, Maharashtra
2007 to 2007
Trainee Jaslok Hospital
Mumbai, Maharashtra
2006 to 2006
Trainee Bombay Hospital
Mumbai, Maharashtra
2006 to 2006
Trainee

Education:

Louisiana Tech University
2010
Master of Science in Cell and Molecular Biology Mumbai University
Mumbai, Maharashtra
2007
Bachelor of Science in Biotechnology

Skills:

Tissue Culture Techniques - Dissection of fish, obtaining organs from fish - Cell and tissue culture techniques (cancer cell lines and mouse embryonic stem cell culture) - Transfection of cell culture with fluorescence proteins; mainly GFP - Efficient handling of Fluorescence microscope for observation of cells - Cryopreservation and maintaining stocks - Perform passage of cell culture and maintain plates Viable cell count in haemocytometer - Plant tissue culture techniques, Mushroom Cultivation Wet Laboratory Techniques - Plasmid DNA preps and PCR amplified product column purification, Plasmid construction, restriction enzyme digestion, ligation, vector design and related techniques used in molecular cloning - Extraction of RNA from fish tissues, Reverse Transcription of RNA to cDNA - Chip technology for gel electrophoresis for quantification of molecules present in PCR product - Sequencing sample preparation and amplification of DNA (PCR), bacterial colony PCR, Gel electrophoresis - Micro-satellite DNA amplification - SDS- PAGE, Western blot and Southern blot analysis - Microbiology techniques such as preparation of agar plates with antibiotics, media/buffer preparation, isolation of pure culture, autoclave, streaking agar plates with efficiency, electroporation Instruments and Software: - Frequent use and good knowledge in MEGA, Oligo primer designing, Biosearch technology for probe designing softwares - Agilent Bioanalyser, Applied Sciences 7500 Fast real time PCR machine, 3130 xl Genetic analyzer for Microsatellite DNA, Any standard PCR machine, NanoDrop 2000, BSL-2 biosafety cabinets/hoods

Vrushalee V . Palsule

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