A hitch pin assembly for connecting a hitch of a towing vehicle (e. g. an agricultural tractor) with a tongue of a towed vehicle (e. g. an agricultural implement) includes a coupling pin having a pinhead and handle at its upper end. The coupling pin is inserted through the hitch and tongue, and is held in place by a collar, which is connected to the lower end of the coupling pin. The collar and the pin are locked together by a locking mechanism which includes a pair of L shaped channels at the lower end of the coupling pin, a pair of locking pins carried by the collar which cooperate with the channels, and a bias spring carried on the collar. The bias spring provides an axial bias force which holds the locking pins in locking seat portions of the channels during normal use. When the tractor and implement are to be disconnected, an axial force sufficient to overcome the bias spring force is applied to the pin and collar, the pin and collar are rotated approximately a quarter turn with respect to one another, and then separated from one another.