Wayne M Barnes

Address:

11 Princeton Ave, Saint Louis, MO 63130

VIN:

2A8GP64L97R193629

Make:

CHRYSLER

Model:

TOWN AND COUNTRY

Year:

2007

US Patent:

6403341, Jun 11, 2002

Filed:

Aug 2, 2001

Appl. No.:

09/920872

Inventors:

Wayne M. Barnes - University City MO 63130
Katherine R. Rowlyk - St. Louis MO

Assignee:

Wayne M. Barnes - University City MO

International Classification:

C12P 1934

US Classification:

435 912, 536 231, 536 2433

Abstract:

The present invention provides a method of performing hot start PCR reactions. The method is based on sequestration of magnesium ions in the form of a precipitate which renders a DNA polymerase inactive until the appropriate time in the PCR reaction when a certain temperature is reached and the magnesium ions are released from the precipitate. Also provided are kits comprising reagents and instructions for amplifying a target nucleic acid by the magnesium precipitate hot start.

US Patent:

6410277, Jun 25, 2002

Filed:

Sep 16, 1997

Appl. No.:

08/931818

Inventors:

Wayne M. Barnes - Chesterfield MO

Assignee:

Takara Shuzo Co., Ltd. - Otsu

International Classification:

C12P 1934

US Classification:

435 912, 435 6

Abstract:

A formulation and kit of thermostable or other DNA polymerases comprising at least one thermostable or other DNA polymerase which lacks 3â-exonuclease activity, and at least one thermostable DNA polymerase exhibiting 3â-exonuclease activity. Also provided is an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyze primer extension.

US Patent:

7393635, Jul 1, 2008

Filed:

May 16, 2002

Appl. No.:

10/147430

Inventors:

Wayne Morris Barnes - University City MO, US

Assignee:

DNA Polymerase Technology, Inc. - University City MO

International Classification:

C12Q 1/68

US Classification:

435 6, 435 911, 435 912

Abstract:

The present invention provides methods of linking nucleic acids without the use of restriction enzymes or any joining enzyme such as ligase. More specifically, the present invention provides methods for cloning or rearranging a double-stranded target DNA which is a PCR product into a double-stranded vector DNA which is a PCR product, said DNAs being amplified using primers that contain at least one ribonucleotide, preferably at or near the respective 3′ ends of the primers, such that RNA-specific cleavage, preferably by RNAse A, will allow release of the primers to create long matching 3′-sticky ends.

US Patent:

7462475, Dec 9, 2008

Filed:

Dec 6, 2004

Appl. No.:

11/005559

Inventors:

Milko B. Kermekchiev - St. Louis MO, US
Wayne M. Barnes - University City MO, US

Assignee:

DNA Poleymerase Technology, Inc. - University City MO

International Classification:

C12N 9/12
C12P 19/34
C07K 14/00

US Classification:

435194, 435183, 435 911, 530350

Abstract:

A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.

US Patent:

7465539, Dec 16, 2008

Filed:

Jun 7, 1995

Appl. No.:

08/483535

Inventors:

Wayne M. Barnes - University City MO, US

Assignee:

Takara Bio, Inc.

International Classification:

C12Q 1/68

US Classification:

435 6, 435 911, 435 912

Abstract:

A formulation of thermostable or other DNA polymerases comprising (a) at least one thermostable or other DNA polymerase which lacks 3′-exonuclease activity, and (b) at least one thermostable DNA polymerase exhibiting 3′-exonuclease activity, wherein the ratio of (a) to (b) is greater than 1 to 1. Also provided is an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyze primer extension, are also provided.

US Patent:

7855055, Dec 21, 2010

Filed:

Oct 21, 2008

Appl. No.:

12/254969

Inventors:

Wayne M. Barnes - University City MO, US

Assignee:

Takara Shuzo Co., Ltd. - Shiga

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

A formulation and kit of thermostable or other DNA polymerases comprising at least one thermostable or other DNA polymerase which lacks 3′-exonuclease activity, and at least one thermostable DNA polymerase exhibiting 3′-exonuclease activity. Also provided is an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyze primer extension.

US Patent:

8470563, Jun 25, 2013

Filed:

Dec 8, 2008

Appl. No.:

12/330201

Inventors:

Milko B. Kermekchiev - University City MO, US
Wayne Morris Barnes - University City MO, US

Assignee:

DNA Polymerase Technology Inc. - St. Louis MO

International Classification:

C12P 19/34

US Classification:

435 911, 435194, 435183, 530350

Abstract:

A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.

US Patent:

20020004203, Jan 10, 2002

Filed:

Feb 6, 2001

Appl. No.:

09/777537

Inventors:

Wayne Barnes - University City MO, US
Milko Kermekchiev - St. Louis MO, US

International Classification:

C12Q001/68
C07H021/04

US Classification:

435/006000, 536/023100, 435/183000, 435/325000, 435/320100

Abstract:

Provided are mutant DNA polymerases having at least one mutation which exhibit substantially reduced polymerase activity at 25 C. when compared to the same DNA polymerases without the at least one mutation and which exhibit normal or near-normal polymerase activity at optimum temperatures when compared to the same DNA polymerases without the at least one mutation. Also provided are amino acid sequences and nucleic acid sequences encoding such DNA polymerases, and vector plasmids and host cells suitable for the expression of these sequences. Also described herein are improved methods for performing polymerase chain reaction (PCR) amplification and other genetic manipulations and analyses using the mutant DNA polymerases of the invention.