Wayne P Fitzmaurice

US Patent:

6344597, Feb 5, 2002

Filed:

Jul 15, 1999

Appl. No.:

09/353787

Inventors:

Wayne P. Fitzmaurice - Vacaville CA

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

A01H 100

US Classification:

800269, 800298, 435 6, 435410, 435468

Abstract:

The present invention relates to novel interspecific hybrid seeds and plants and to a method of producing interspecific Nicotiana hybrids having enhanced properties for biomass and the production of recombinant proteins using a viral vector system.

US Patent:

6468745, Oct 22, 2002

Filed:

Jul 21, 1999

Appl. No.:

09/359304

Inventors:

Wayne P. Fitzmaurice - Vacaville CA
John A. Lindbo - Vacaville CA
Hal S. Padgett - Vacaville CA
Gregory P. Pogue - Vacaville CA

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

C12Q 168

US Classification:

435 6, 435 691, 435 914, 435410, 435 5, 435468, 435440, 4352351, 435441, 435446, 536 231

Abstract:

The present invention relates to a method for using viral vectors to bear populations of sequence variants and using plant hosts to select the sequences that exhibit the desired traits.

US Patent:

6656726, Dec 2, 2003

Filed:

May 4, 2000

Appl. No.:

09/565616

Inventors:

Wayne P. Fitzmaurice - Vacaville CA
Gregory P. Pogue - Vacaville CA
John A. Lindbo - Vacaville CA

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

C12N 1500

US Classification:

4353201, 435410, 435419, 435468

Abstract:

The present invention provides nucleic acid sequences having an altered viral movement protein and 126/183 kDa replicase proteins further characterized in its ability to stabilize a transgene contained in a virus that expresses the altered movement protein. The present invention also provides viral vectors expressing the altered movement protein, cells transformed with the vectors, and host plants infected by the viral vectors.

US Patent:

7056740, Jun 6, 2006

Filed:

Jan 31, 2003

Appl. No.:

10/356708

Inventors:

Hal S. Padgett - Vacaville CA, US
Andrew A. Vaewhongs - Vacaville CA, US
Fakhrieh S. Vojdani - Davis CA, US
Mark L. Smith - Davis CA, US
John A. Lindbo - Vacaville CA, US
Wayne P. Fitzmaurice - Vacaville CA, US

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

C12N 15/00

US Classification:

435440, 435 691

Abstract:

We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.

US Patent:

7132588, Nov 7, 2006

Filed:

Jul 21, 2003

Appl. No.:

10/624193

Inventors:

Wayne P. Fitzmaurice - Vacaville CA, US
Gregory P. Pogue - Vacaville CA, US
John A. Lindbo - Vacaville CA, US

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

A01H 1/00
A01H 11/00
C12N 15/05
C07H 21/02
C07H 21/04

US Classification:

800278, 800277, 800295, 536 231, 536 234

Abstract:

The present invention provides nucleic acid sequences having an altered viral movement protein and 126/183 kDa replicase proteins further characterized in its ability tostabilize a transgene contained in a virus that expresses the altered movement protein. The present invention also provides viral vectors expressing the altered movement protein, cells transformed with the vectors, and host plants infected by the viral vectors.

US Patent:

7217514, May 15, 2007

Filed:

Jul 25, 2002

Appl. No.:

10/206030

Inventors:

Hal S. Padgett - Vacaville CA, US
John A. Lindbo - Vacaville CA, US
Wayne P. Fitzmaurice - Vacaville CA, US

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07H 21/00

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243, 536 2433, 536 253

Abstract:

We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

US Patent:

7235386, Jun 26, 2007

Filed:

Jul 25, 2002

Appl. No.:

10/205772

Inventors:

Hal S. Padgett - Vacaville CA, US
John A. Lindbo - Vacaville CA, US
Wayne P. Fitzmaurice - Vacaville CA, US

Assignee:

Large Scale Biology Corporation - Vacaville CA

International Classification:

C12P 19/34
C12Q 1/68
C07H 21/02
C07H 21/04
C07H 21/00

US Classification:

435 912, 435 6, 435 911, 536 231, 536 243, 536 2433, 536 253

Abstract:

We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

US Patent:

7582423, Sep 1, 2009

Filed:

Oct 25, 2002

Appl. No.:

10/280913

Inventors:

Hal S. Padgett - Vacaville CA, US
John A. Lindbo - Vacaville CA, US
Wayne P. Fitzmaurice - Vacaville CA, US

Assignee:

Novici Biotech LLC - Vacaville CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 6, 435 912, 536 231

Abstract:

We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.