Wei M He

US Patent:

20130165636, Jun 27, 2013

Filed:

Dec 19, 2012

Appl. No.:

13/719785

Inventors:

The Regents of The University of California - Oakland CA, US
Wei He - Davis CA, US
Kit S. Lam - Davis CA, US
Paul Henderson - Dublin CA, US
Matt Coleman - Oakland CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07K 17/02

US Classification:

530359, 435 681

Abstract:

The present invention provides a nanodisc with a membrane scaffold protein. The nanodisc includes a membrane scaffold protein, a telodendrimer and a lipid. The membrane scaffold protein can be apolipoprotein. The telodendrimer has the general formula PEG-L-D-(R), wherein D is a dendritic polymer; L is a bond or a linker linked to the focal point group of the dendritic polymer; each PEG is a poly(ethylene glycol) polymer; each R is and end group of the dendritic polymer, or and end group with a covalently bound hydrophobic group, hydrophilic group, amphiphilic compound, or drug; and subscript n is an integer from 2 to 20. Cell free methods of making the nanodiscs are also provided.