The present invention is directed to methods and compositions that are effective in the inhibition of viral replication. In particular, the methods and compositions are effective at interfering with the activity of host cell proteins required in viral replication. For example, an embodiment of the invention is directed to inhibition of flavivirus replication wherein the replication is effected by changing the normal interactions of the host cell protein TIAR or TIA-1.
Scheffer Tseng - Pinecrest FL, US Hua He - Miami FL, US Wei Li - Miami FL, US
International Classification:
C12N 5/06 C12N 5/08
US Classification:
435325000, 435366000
Abstract:
An ex vivo method of expanding animal cells whose differentiation state is controllable by modulating TGF-β signaling includes the steps of: (a) providing an animal subject having cells with a first phenotype; (b) isolating the cells from the animal subject; (c) placing the cells in an ex vivo culture system including a culture vessel having at least one surface and a medium in contact with the at least one surface, the medium being essentially free of intact amniotic membrane and feeder cells; and (d) culturing the cells in the medium under conditions which downregulate TGF-β signaling in the cells and allow the cells to proliferate while maintaining the first phenotype.
Methods And Compositions For Inhibition Of Viral Replication
The present invention is directed to methods and compositions that are effective in the inhibition of viral replication. In particular, the methods and compositions are effective at interfering with the activity of host cell proteins required in viral replication. For example, an embodiment of the invention is directed to inhibition of flavivirus replication wherein the replication is affected by changing the normal interactions of the host cell protein TIAR or TIA-1.
Pirouz M. Daftarian - Miami FL, US Paolo Serafini - Miami Shores FL, US Vance Paul Lemmon - Miami FL, US Angel Kaifer - Coral Gables FL, US Victor L. Perez - Pinecrest FL, US Wei Li - Miami FL, US Bonnie Beth Blomberg - Coral Gables FL, US
Nanoparticle-based vaccines, compositions, kits and methods are used for the effective delivery of one or more antigens in vivo for vaccination and antibody (e.g., monoclonal antibody) production, and for the effective delivery of peptides, proteins, siRNA, RNA or DNA to PAPCs or MHC class II positive cells (e.g. tumor cells). Antigens may be, for example, DNA that results in expression of the gene of interest and induction of a robust and specific immune response to the expressed protein in a subject (e.g., mammal). Antigens may also be immunogenic peptides or polypeptides that are processed and presented. In one embodiment, a nanoparticle -based method to deliver antigens in vivo as described herein includes injection of a vaccine composed of a DNA encoding at least one antigen, or at least one antigenic peptide or polypeptide conjugated to a charged dendrimer (e.g., PADRE-derivatized dendrimer) that is also conjugated to a T helper epitope (e.g., PADRE). Negatively-charged plasmids bind naturally to a positively-charged PADRE-dendrimer, while peptide or polypeptide antigens can be chemically linked to the PADRE-dendrimer if they are not negatively-charged. Alternatively, negatively-charged dendrimers may be used. The compositions, kits, vaccines and methods described herein have both prophylactic and treatment applications, i.e., can be used as a prophylactic to prevent onset of a disease or condition in a subject, as well as to treat a subject having a disease or condition. A vaccine as described herein can be used to mount an immune response against any infectious pathogen or cancer.
- Norcross GA, US Wei Li - Norcross GA, US Yuan Di - Norcross GA, US Annie You - Norcross GA, US Low Wee Fong - Singapore, SG
International Classification:
A61L 9/22 A61L 9/20 A61L 2/10 A61L 2/24 A61L 2/26
Abstract:
A germicidal device includes a purification/disinfection and LED lighting member configured to generate UVC light and negative air ions for destroying microorganisms and removing odors in a space in which the purification and sterilization member is placed; a sensing member configured to detect at least one event occurred in said space in real time; and a microcontroller unit coupled with the purification and sterilization member and the sensing member for controlling operations of the purification and sterilization member in a respective state in accordance with the at least one event occurred in said space and energy saving. The UVC light has one or more wavelengths in a range of about 100-400 nm.
Separable Microneedle Arrays For Sustained Release Of Drug
- Atlanta GA, US Richard N. TERRY - Conyers GA, US Wei LI - Atlanta GA, US
International Classification:
A61M 37/00 A61K 31/57 A61K 9/46 A61K 9/00
Abstract:
Separable microneedle arrays and microneedle patches are provided that may achieve sustained release of drug. The microneedle arrays may include one or more features that facilitate separation of the microneedles, such as a bubble structure. The microneedle arrays may include an effervescent material.
Quantitative Ligandomics For Systematic Identification Of Therapeutic Ligands
The present invention is directed to methods for systematic identification of cellular ligands, disease-associated ligands, age-related ligands and receptor-specific ligands. Disease-associated ligands are promising targets to develop novel ligand-based therapies. The methods are broadly applicable to any type of cells or diseases in in vitro and in vivo settings. This invention further used the methods to identify Scg3 as a disease-related angiogenic factor for the therapy of diabetic retinopathy, diabetic macular edema, proliferative diabetic retinopathy, vascular age-related macular degeneration, diabetic foot and cancers.
UnityPoint ClinicsUnitypoint Clinic Cardiology 202 10 St SE STE 225, Cedar Rapids, IA 52403 3193647101 (phone), 3193631993 (fax)
Mercycare Community PhysiciansMercy Internists Associates Of Iowa 543 7 St SE, Cedar Rapids, IA 52401 3198617600 (phone), 3198617614 (fax)
Education:
Medical School Peking Union Med Coll, Beijing, Beijing, China Graduated: 1996
Procedures:
Nutrition Therapy
Conditions:
Diabetes Mellitus (DM) Disorders of Lipoid Metabolism Hypertension (HTN) Hypothyroidism
Languages:
English
Description:
Dr. Li graduated from the Peking Union Med Coll, Beijing, Beijing, China in 1996. She works in Cedar Rapids, IA and 1 other location and specializes in Cardiovascular Disease and Internal Medicine. Dr. Li is affiliated with Marengo Memorial Hospital, Mercy Medical Center and St Lukes Hospital.
Dr. Li graduated from the Capital Univ of Med Scis, Training Ctr of Gen Prac, Beijing City, China in 1992. She works in Smithtown, NY and specializes in Cardiovascular Disease. Dr. Li is affiliated with Good Samaritan Hospital Medical Center, Saint Catherine Of Siena Medical Center and Southside Hospital.
Dr. Li graduated from the Xuzhou Med Coll, Xuzhou City, Jiangsu, China in 1985. He works in Plymouth, MA and specializes in Internal Medicine. Dr. Li is affiliated with Beth Israel Deaconess Hospital-Plymouth.
Dr. Li graduated from the SUNY Downstate Medical Center College of Medicine in 2005. He works in Belleville, NJ and 2 other locations and specializes in Allergy & Immunology and Internal Medicine. Dr. Li is affiliated with Clara Maass Medical Center, Hackensack University Medical Center Mountainside, Meadowlands Hospital Medical Center, New York Eye & Ear Infirmary Of Mount Sinai, Newton
Portsmouth Anesthesia AssociatesPortsmouth Anesthesia Associates PC 5818 Harbour Vw Blvd, Suffolk, VA 23435 7573997451 (phone), 7573991158 (fax)
Education:
Medical School Tianjin Med Univ, Tianjin City, Tianjin, China Graduated: 1982
Languages:
English
Description:
Dr. Li graduated from the Tianjin Med Univ, Tianjin City, Tianjin, China in 1982. He works in Portsmouth, VA and 2 other locations and specializes in Anesthesiology. Dr. Li is affiliated with Bon Secours DePaul Medical Center and Bon Secours Maryview Medical Center.
May 2010 to 2000 Staff Geotechnical EngineerGeorgia Institute of Technology
Jan 2006 to Aug 2010 Research AssistantTianjin Port Engineering Institute (TPEI)
Aug 1999 to Jul 2004 Geotechnical EngineerHohai University
Sep 1996 to Jul 1999 Research Assistant
Education:
Georgia Institute of Technology Atlanta, GA Jun 2010 Ph.D. in Civil EngineeringHohai University Apr 1999 M.S. in Civil EngineeringShandong Polytechnic University Jul 1996 B.S. in Civil Engineering