Wendell A Lim

US Patent:

7879590, Feb 1, 2011

Filed:

Mar 13, 2008

Appl. No.:

12/047767

Inventors:

Wendell A. Lim - San Francisco CA, US
Caleb J. Bashor - San Francisco CA, US

Assignee:

Regents of the University of California - Oakland CA

International Classification:

C12N 1/20
C12P 21/06

US Classification:

4352542, 435 691

Abstract:

Synthetic scaffold interactions and scaffold-mediated feedback loops are used to engineer MAPK signaling responses in cells.

US Patent:

20050004347, Jan 6, 2005

Filed:

Jul 3, 2003

Appl. No.:

10/613380

Inventors:

Wendell Lim - San Francisco CA, US
John Dueber - San Francisco CA, US
Brian Yeh - San Francisco CA, US

International Classification:

G06F019/00
G01N033/48
G01N033/50
C12N009/00

US Classification:

530350000, 702019000, 435183000

Abstract:

Protein logic gates are made from autoregulated fusion proteins comprising an output domain and a plurality of input domains, wherein at least one of the input domains is heterologous to the output domain, and the input domains interact with each other to allosterically and external, ligand-dependently regulate the output domain. The output domain may be constitutively active, and in the absence of the ligand, the input domains interact to inhibit the output domain. The activity of the output domain is user discretionary, and may include activities that are catalytic, label-generative, metabolic-regulative, apototic, specific-binding, etc. Multiple input domains can cooperatively regulate the fusion protein in a wide variety of functionalities, including as an OR-gate, an AND-gate, and an AND-NOT-gate. The gates may be incorporated into cells and therein used to modulate cell function.

US Patent:

20140068797, Mar 6, 2014

Filed:

Mar 15, 2013

Appl. No.:

13/842859

Inventors:

Jennifer A. Doudna - Berkeley CA, US
Martin Jinek - Berkeley CA, US
Emmanuelle Charpentier - Berkeley CA, US
Krzysztof Chylinski - Berkeley CA, US
James Harrison Doudna Cate - Berkeley CA, US
Wendell Lim - San Francisco CA, US
Lei Qi - Albany CA, US

Assignee:

UNIVERSITY OF VIENNA - Vienna
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12N 15/90
C12N 15/113
C12N 9/22

US Classification:

800 18, 536 231, 4353201, 435199, 435325, 435243, 4352523, 435419, 4352572, 435349, 435352, 435353, 435354, 435363, 435366, 435462, 435 9153, 435375, 536 245, 506 16, 800298, 800 13, 800 19, 514 44 R, 424 9321, 424 932

Abstract:

The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

US Patent:

20220213452, Jul 7, 2022

Filed:

Aug 25, 2021

Appl. No.:

17/412047

Inventors:

- Oakland CA, US
Wendell A. Lim - San Francisco CA, US
Chia-Yung Wu - San Francisco CA, US

International Classification:

C12N 9/12
C07K 14/72
C07K 1/36
C07K 2/00
C07K 19/00

Abstract:

The present disclosure provides conditionally active, heterodimeric polypeptides. The conditionally active, heterodimeric polypeptides are active in the presence of a dimerizing agent that induces dimerization of the polypeptides of the heterodimer. A conditionally active, heterodimeric polypeptide of the present disclosure is useful in a variety of research and treatment methods, which are also provided.

US Patent:

20230078007, Mar 16, 2023

Filed:

Feb 19, 2021

Appl. No.:

17/800872

Inventors:

- Oakland CA, US
Devan SHAH - San Franisco CA, US
Wendell A. LIM - San Francisco CA, US

International Classification:

A61K 35/17
C12N 5/0783
C12N 15/11
C12N 9/22
C12N 15/62
C12N 9/02
A61P 35/00
A61K 35/15
C07K 16/28
C07K 14/725
A61K 38/19
A61K 38/44
A61K 38/48
C07K 16/32

Abstract:

Provided herein is an in-series synthetic receptor circuit for dual-antigen AND-gate control over expression of a therapeutic payload by engineered cells. In some embodiments, the circuit may be composed of a first binding-triggered transcriptional switch, a second binding-triggered transcriptional switch and a therapeutic payload (e.g., a chimeric antigen receptor), where binding of the first binding-triggered transcriptional switch to a first antigen activates expression of the second binding-triggered transcriptional switch, and binding of the second binding-triggered transcriptional switch to a second antigen activates expression of the therapeutic payload. If the cell is an immune cell and the therapeutic payload is a chimeric antigen receptor, then the immune cell may be activated by binding of the chimeric antigen receptor to a third antigen. Methods of treatment using the cell also provided.

US Patent:

20230051989, Feb 16, 2023

Filed:

Sep 14, 2022

Appl. No.:

17/932259

Inventors:

- Oakland CA, US
James Onuffer - Alameda CA, US
Wendell A. Lim - San Francisco CA, US

International Classification:

A61K 35/17
A61K 47/68
C07K 14/705
C07K 16/18
C07K 16/28
C07K 14/725
C12N 9/90
C12N 9/14
C12N 5/0783

Abstract:

The present disclosure provides a heterodimeric, conditionally active chimeric antigen receptor (CAR), and a nucleic acid comprising a nucleotide sequence encoding the CAR. The present disclosure provides cells genetically modified to produce the CAR. A CAR of the present disclosure can be used in various methods, which are also provided.

US Patent:

20210275588, Sep 9, 2021

Filed:

Jul 9, 2019

Appl. No.:

17/258039

Inventors:

- Oakland CA, US
Xiao Huang - Gushi, CN
Ryan Chang - San Francisco CA, US
Jasper Z. Williams - San Francisco CA, US
Wendell A. Lim - San Francisco CA, US

International Classification:

A61K 35/17
A61P 35/00
A61K 47/69
C07K 14/55

Abstract:

The present disclosure provides polymeric particles comprising biomolecules of interest attached thereto, methods for using the same, and methods for making the same. The surface of the polymeric particles can be functionalized by attaching multiple different biomolecules of interest in a desired ratio for co-presentation. In addition, the polymeric particles may also encapsulate bio-molecules, such as, therapeutic nucleic acids, peptide and/or polypeptides for release in vivo. The present disclosure also provide synthetic particles and methods for enhancing proliferation of CAR-T cells. Additionally, the present disclosure provide biomolecule-coated films and methods.

US Patent:

20200331985, Oct 22, 2020

Filed:

Nov 15, 2018

Appl. No.:

16/763524

Inventors:

- Oakland CA, US
- New York NY, US
Wendell A. Lim - San Francisco CA, US
Kole T. Roybal - San Francisco CA, US
Gary Struhl - New York NY, US

International Classification:

C07K 14/755
C07K 14/705
C07K 14/72
C07K 16/28
G01N 33/50
G01N 33/68
C07K 14/59

Abstract:

Provided are chimeric polypeptides which modulate various cellular processes following cleavage of a force sensor cleavage domain, including non-Notch force sensor cleavage domains, induced upon binding of a specific binding member of the chimeric polypeptide with its binding partner. Methods of using force sensor cleavage domain-containing chimeric polypeptides to modulate cellular functions, including e.g., modulation (including induction or repression) of gene expression, are also provided. Nucleic acids encoding the subject chimeric polypeptides and associated expression cassettes and vectors as well as cells that contain such nucleic acids and/or expression cassettes and vectors are provided. Also provided, are methods of monitoring cell-cell signaling and method of treating a subject using the described components, as well as kits for practicing the subject methods.