William W Alexander

Address:

1419 31 Ave S APT B, Seattle, WA 98144

VIN:

1FTNE14W87DA89721

Make:

FORD

Model:

E-SERIES CARGO

Year:

2007

US Patent:

7479276, Jan 20, 2009

Filed:

Feb 22, 2000

Appl. No.:

09/914046

Inventors:

Liang Xu - Arlington VA, US
Cheng-Cheng Huang - Arlington VA, US
William Alexander - Rockville MD, US
WenHua Tang - Arlington VA, US
Esther H. Chang - Chevy Chase MD, US

Assignee:

SynerGene Therapeutics, Inc. - Washington DC
Georgetown University - Washington DC

International Classification:

A61K 39/00
A61K 39/398
A61K 31/70
A61K 31/711

US Classification:

4241411, 4241781, 4241791, 424450, 514 44

Abstract:

Nucleic acid-immunoliposome compositions useful as therapeutic agents are disclosed. These compositions preferably comprise (i) cationic liposomes, (ii) a single chain antibody fragment which binds to a transferrin receptor, and (iii) a nucleic acid encoding a wild type p53. These compositions target cells which express transferrin receptors, e. g. , cancer cells. These compositions can be used therapeutically to treat persons or animals who have cancer, e. g. , head and neck cancer, breast cancer or prostate cancer.

US Patent:

20050063950, Mar 24, 2005

Filed:

Apr 8, 2004

Appl. No.:

10/820144

Inventors:

Esther Chang - Potomac MD, US
Kathleen Pirollo - Rockville MD, US
Liang Xu - Arlington VA, US
William Alexander - Rockville MD, US

Assignee:

Georgetown University - Washington DC
SynerGene Therapeutics, Inc. - Washington DC

International Classification:

C12P021/04
A61K048/00
C12N015/867
C12N015/861

US Classification:

424093200, 435456000

Abstract:

The present invention relates to gene transfer and gene therapy technology. More specifically, the invention provides compositions and methods for targeted virus delivery. The method utilizes a method of mixing the virus, which may be a recombinant virus which will express a protein of interest or a nucleic acid of interest, with a cell-targeting ligand, e.g., transferrin. The virus and ligand are mixed without crosslinkers or agents which would covalently bond the virus and ligand. This simple mixing causes less inactivation than chemically linking the ligand to the virus and therefore results in a more active therapeutic composition than obtained by methods which utilize crosslinking agents.

US Patent:

20070065432, Mar 22, 2007

Filed:

Nov 2, 2006

Appl. No.:

11/591478

Inventors:

Liang Xu - Arlington VA, US
Cheng-Cheng Huang - Arlington VA, US
William Alexander - Rockville MD, US
WenHua Tang - Arlington VA, US
Esther Chang - Chevy Chase MD, US

Assignee:

SynerGene Therapeutics, Inc. - Washington DC
Georgetown University - Washington DC

International Classification:

A61K 48/00
A61K 39/395
A61K 9/127

US Classification:

424143100, 424450000, 514044000

Abstract:

A targeted vector allowing enhanced gene transfer to human hepato-cellular carcinoma (HCC) cells in vitro was developed using cationic liposomes covalently conjugated with the mAb AF-20. This high affinity antibody recognizes a rapidly internalized 180 kDa cell surface glycoprotein which is abundantly expressed on the surface of human HCC and other cancer cells. Quantitative binding analysis of liposomes with target cells by flow cytometry showed specific association of mAb-targeted liposomes with human HCC cells. Using mAb-targeted cationic liposomes containing 20% DOTAP, in the presence or absence of serum, gene expression in HuH-7 cells was enhanced up to 40-fold as compared to liposomes conjugated with an isotype-matched non-relevant control antibody. Transfection specificity was not observed in a control cell line that does not express the antigen recognized by mAb AF-20. This study demonstrates that cationic liposome foormulations can be targeted with monclonal antibodies (mAbs) to enhance specific in vitro gene delivery and expression in the presence or absence of serum.

US Patent:

20080213223, Sep 4, 2008

Filed:

Oct 31, 2007

Appl. No.:

11/980791

Inventors:

Esther H. Chang - Potomac MD, US
Kathleen F. Pirollo - Rockville MD, US
Liang Xu - Arlingon VA, US
William Alexander - Rockville MD, US

Assignee:

SynerGene Therapeutics, Inc. - Washington DC

International Classification:

A61K 48/00
C12N 15/861
A61P 35/00

US Classification:

424 932, 435456

Abstract:

The present invention relates to gene transfer and gene therapy technology. More specifically, the invention provides compositions and methods for targeted virus delivery. The method utilizes a method of mixing the virus, which may be a recombinant virus which will express a protein of interest or a nucleic acid of interest, with a cell-targeting ligand, e.g., transferrin. The virus and ligand are mixed without crosslinkers or agents which would covalently bond the virus and ligand. This simple mixing causes less inactivation than chemically linking the ligand to the virus and therefore results in a more active therapeutic composition than obtained by methods which utilize crosslinking agents.

US Patent:

20110166550, Jul 7, 2011

Filed:

Apr 27, 2009

Appl. No.:

12/988353

Inventors:

William Allan Alexander - Mercer Island WA, US
Paul D. Bishop - Fall City WA, US
Steve R. Carter - Edmonds WA, US
Alisa M. Littau - Woodinville WA, US

Assignee:

ZYMOGENETICS, INC. - Seattle WA

International Classification:

A61M 37/00
A61B 17/00

US Classification:

604518, 606 1, 604500

Abstract:

Provided is a surgical applicator and applicator tip configured to attach in fluid communication, for the delivery of beneficial fluids. The applicator tip may also be configured to reversibly attach, in fluid communication, with a pledget. For example, the applicator tip may include male or female threads, and the pledget has threads that are complementary to the thread configuration of the tip. The applicator tip may further comprise at least one fluid transfer lumen for transfer of a fluid from the tip to the pledget. In one embodiment of the invention, the tip includes male threads and the pledget includes a pledget attachment portion. There are also provided methods for connecting the tip to the pledget. In one embodiment, a tip comprising male threads is attached to the pledget, comprising a pledget attachment portion, by tapping or pressing the tip into the pledget attachment portion. There are also provided methods of use for the attached tip and pledget, including debridement, tissue translocation, delivery of fluid substances, absorption of fluid substances and combinations thereof. In a particular embodiment, the applicator places a thrombin solution into the attached pledget for delivery of an active hemostatic agent to a tissue site.

US Patent:

62799096, Aug 28, 2001

Filed:

Aug 17, 1998

Appl. No.:

9/135383

Inventors:

William Whitney Alexander - Seattle WA
Richard J. Tait - Seattle WA

Assignee:

Cranium, Inc. - Seattle WA

International Classification:

A63F 100

US Classification:

273249

Abstract:

A game kit (20) includes a game playing surface (22) marked with a plurality of indicia (42), (44), (46), (48), and (50). The game kit also includes a plurality of markers (26a-26d) for positioning on indicia of the playing surface. A first deck of cards (24a) including a plurality of cards (56) is also included. Each card bearing instructions (64) for a player to carry out an activity. The activity instructions of the cards in the first deck each being associated with a first core or cluster of human competencies selected from the group of factual knowledge competencies, audio/video performance competencies, two and/or three-dimensional graphic competencies, and spelling/vocabulary competencies. The plurality of cards contained in the first deck including at least a first subset of cards directed to a first activity type associated with the first core or cluster of competency(s) and a second subset of cards directed to a second activity type, distinct from the first activity type, associated with the first core or cluster of competency(s). The game also includes a second deck of cards having a plurality of cards bearing instructions for a player to carry out an activity.