William Frank Dougherty

US Patent:

55321429, Jul 2, 1996

Filed:

Feb 12, 1993

Appl. No.:

8/021603

Inventors:

Stephen A. Johnston - Dallas TX
William G. Dougherty - Philomath OR

Assignee:

Board of Regents, the University of Texas System - Austin TX
State of Oregon, The, Acting By and Through the Oregon State System of
Higher Education, on Behalf of Oregon State University - Corvallis OR

International Classification:

C12N 952
C12P 2100

US Classification:

435 691

Abstract:

Methods for isolation and purification of recombinant proteins are described. Fusion proteins incorporating a cleavage site sensitive to proteolysis by a plant virus proteinase may be cleaved from carrier proteins to provide high yields of protein product. Methods employing a plant virus proteinase to cleave expressed fusion proteins are particularly suitable for obtaining proteolytically sensitive polypeptides in the presence of added cell protease inhibitors. Also disclosed are recombinant vectors useful for overproducing plant virus proteinases in a suitable host.