William Mark Knowles

US Patent:

7505063, Mar 17, 2009

Filed:

Feb 16, 2005

Appl. No.:

11/059494

Inventors:

Rodney Bastedo - Stratford CT, US
Dario L. Cavallero - Pittsfield MA, US
William D. Knowles - Pittsfield MA, US
Brian D. Brazeau - N. Adams MA, US

Assignee:

Cavallero Plastics, Inc. - Pittsfield MA

International Classification:

H04N 7/18
H04N 5/225
B08B 9/02
F16L 45/00
F16L 55/44

US Classification:

348 84, 348373, 378 60, 1510405

Abstract:

A mount for traveling within a pipe to center a tool, such as a camera, within the pipe. Inner and outer concentric cylinders are slidably nested. Pivoted leg pairs have one end connected to the outer cylinder and another end connected to the inner cylinder. The pivoted leg pairs move radially inward or outward as the inner and outer cylinder move axially with respect to each other. A spring biases wheels attached to the distal end of the pivoted leg pairs radially outward. The mount centers the inner and outer cylinder within the pipe and automatically adjusts to different diameters of pipes. The mount overcomes obstructions within the pipe and is easily retrievable. The interior of the pipe may be inspected or worked upon.

US Patent:

47242027, Feb 9, 1988

Filed:

Dec 12, 1983

Appl. No.:

6/560462

Inventors:

Nanibhushan Dattagupta - New Haven CT
Peter M. M. Rae - Hamden CT
William J. Knowles - Hamden CT
Donald M. Crothers - Northford CT

Assignee:

Molecular Diagnostics, Inc.

International Classification:

C12Q 168
G01N 3353
G01N 33543

US Classification:

435 6

Abstract:

A detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable, single or double stranded nucleic acid portion, the non-hybridizable portion preferably including a recognition site for a particular protein.

US Patent:

47771291, Oct 11, 1988

Filed:

Oct 19, 1984

Appl. No.:

6/662858

Inventors:

Nanibhushan Dattagupta - New Haven CT
Peter M. M. Rae - Hamden CT
William J. Knowles - Hamden CT
Donald M. Crothers - Northford CT

Assignee:

Molecular Diagnostics, Inc. - West Haven CT

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

A nucleic acid detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable, single or double stranded nucleic acid portion, the non-hybridizable portion preferably including a recognition site for binding by a particular protein. Such recognition site can be a region of singly or doubly stranded nucleic acid specific for a particular nucleic acid binding protein such as lac repressor protein or can be a modified nucleic acid region such as a unique antigenic determinant introduced by interaction of the region with a modifier compound such as an intercalating agent or a platinum-containing ligand. The probe-binding protein can be labeled for ease of detection and in the case of an antigenic determinant binding site can be labeled antibody.

US Patent:

48085200, Feb 28, 1989

Filed:

Mar 15, 1985

Appl. No.:

6/712481

Inventors:

Nanibhushan Dattagupta - New Haven CT
William J. Knowles - Hamden CT

Assignee:

Molecular Diagnostics, Inc. - West Haven CT

International Classification:

C12Q 168
C12P 1934

US Classification:

435 6

Abstract:

A method is provided of making an oligonucleotide which comprises hybridizing (a) a shorter fragment of the desired oligonucleotide with (b) a nucleic acid fragment longer than (a) and complementary to the desired oligonucleotide, one of (a) and (b) is linked to an organic radical which differentiates its physical properties relative to the other of (a) and (b), contacting the hybridized material with an enzyme and nucleoside triphosphates whereby the shorter fragment is extended in one direction until it is substantially coterminal with the complementary nucleic acid fragment, denaturing the hybridized material and separating lengthened (a) from (b).

US Patent:

51660539, Nov 24, 1992

Filed:

Feb 23, 1990

Appl. No.:

7/485100

Inventors:

Edward D. Huguenel - Guilford CT
William J. Knowles - Guilford CT

Assignee:

Miles Inc. - Elkhart IN

International Classification:

G01N 33571

US Classification:

435 736

Abstract:

A method for determining the adequacy of a cervical or urethral test specimen collected for an immunological assay to detect the presence of Chlamydia trachomatis. Cells present in the specimen are disrupted to expose a substance present in or on C. triachomatis that is detectable by immunological reaction. In order to determine if the test specimen contains an adequate amount of the cervical or urethral cell type that serves as a host cell for C. trachomatis, the disrupted specimen is also reacted with an immunological reagent that produces a detectable complex upon specific binding with a substance present in or on columnar epithelial cells. The present invention therefore provides a control reaction that verifies the adequacy of the collected test specimen and thereby increases the confidence that a negative test result for the presence of Chlamydia indicates the absence of a C. trachomatis infection in the patient.

US Patent:

47481110, May 31, 1988

Filed:

Mar 12, 1984

Appl. No.:

6/588858

Inventors:

Nanibhushan Dattagupta - New Haven CT
William J. Knowles - Hamden CT
Vincent T. Marchesi - Guilford CT
Donald M. Crothers - Northford CT

Assignee:

Molecular Diagnostics, Inc. - West Haven CT

International Classification:

G01N 5300
G01N 3353
G01N 33543
C12Q 168

US Classification:

435 7

Abstract:

A protein is covalently coupled to a 3'terminal end of a nucleic acid which carries several labels. In an assay the protein will specifically recognize some component of a test system; in an immunoassay the protein can be Protein A which will recognize the FC portion of IgG which is bound to an unknown antigen if present in the test sample.

US Patent:

46580220, Apr 14, 1987

Filed:

Sep 27, 1985

Appl. No.:

6/779730

Inventors:

William J. Knowles - Hamden CT
Vincent T. Marchesi - Gulford CT

Assignee:

Molecular Diagnostics, Inc. - West Haven CT

International Classification:

A61K 3900
G01N 3356

US Classification:

530402

Abstract:

Binding of a particular protein by an antibody reagent involving denaturation of the protein and use of an antibody reagent specific for binding a linear peptide epitope therein. Denaturation by chemical or physical means effectively exposes or enhances the exposure of the linear peptide epitope for binding by the antibody reagent which is preferably raised against a synthetic peptide immunogen. The technique is particularly useful in performing immunoassays for protein analytes, such as a glycosylated protein, in aqueous test samples.

US Patent:

45986002, Jul 8, 1986

Filed:

Dec 5, 1983

Appl. No.:

6/558400

Inventors:

William C. Knowles - Glastonbury CT

Assignee:

United Technologies Corporation - Hartford CT

International Classification:

F16H 114
F02C 720

US Classification:

74423

Abstract:

The intermediate case for turbo fan engines has a bearing support cone extending forwardly from the support flange in alignment with the struts in the case to provide a secure support for the #2 bearing for the low pressure shaft. To accommodate the power shaft assembly which extends through the cone the latter has a bulge in which is mounted the support cylinder for the bearings for the power shaft, this bulge providing a support structure for the cylinder and clearance for a bevel gear on the power shaft journaled in the cylinder.