William H Rust

US Patent:

20020068316, Jun 6, 2002

Filed:

Dec 5, 2000

Appl. No.:

09/730700

Inventors:

William Rust - Las Vegas NV, US
Janice Huff - Henderson NV, US
George Plopper - Henderson NV, US

International Classification:

G01N033/48

US Classification:

435/040500

Abstract:

A method for measuring both the anti-migratory and cytotoxic effects of anticancer drugs on cancer tissues measuring the chemosensitivity of human biopsies to therapeutic drugs. Single cell suspension preparations of biopsy tissue is exposed to anticancer drugs and introduced into the first chamber of a migration plate separated from a second chamber by a porous membrane opaque to radiation. A migration stimulus is added to the second chamber. Migrated cells on the side of the membrane facing the second chamber are labeled with a live-cell fluorescent indicator. Non-migrated cells on the side of the membrane facing the first chamber are labeled with a fluorescent indicator of cell death. The emitted fluorescence of both the migrated cells and the non-viable cells is quantified in a fluorescence plate reader. The comparative intensity of fluorescence is an indicator of the susceptibility of the cells to the cytotoxic properties of the drug.