Wlodzimierz E Dembinski from 7 Marine Dr, Buffalo, NY 14202, age 77, Phone: 7168569768

Wlodzimierz Dembinski Phones & Addresses

Method For The Large Scale Purification Of Human Fibroblast Interferon
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US Patent:

46580174, Apr 14, 1987
Filed:

Feb 14, 1984
Appl. No.:

6/579994
Inventors:

Wlodzimierz E. Dembinski - Buffalo NY
Eugene Sulkowski - Buffalo NY
Assignee:

Health Research, Inc. (Roswell Park Division) - Buffalo NY
International Classification:

C07K 320
C07K 1526
A61K 4502
US Classification:

530351
Abstract:

A process for the large scale purification of human fibroblast interferon produced by human diploid fibroblasts under superinduction conditions characterized by a sequence of four chromatographic steps namely: (1) separation on CM-agarose; (2) separation on ConA-agarose; (3) separation on phenyl-agarose; and (4) elimination of ethylene glycol using CM-agarose which process yields a final overall recovery not less than 60% of the crude interferon starting material and having a specific activity not less than 1. times. 10. sup. 8 units per mg of protein.