Xiangdong Ma

US Patent:

20090017496, Jan 15, 2009

Filed:

Oct 11, 2005

Appl. No.:

11/667158

Inventors:

Yangao Ma - Hubei, CN
Xiangdong Ma - North Andover MA, US

International Classification:

C12P 21/04
C12N 15/11
C12N 15/00
C07K 14/00

US Classification:

435 694, 536 2351, 530303, 4353201

Abstract:

A recombinant genetic construct is provided that includes at least two expression cassettes. Each cassette encodes for a chain or subunit of a target protein. The genetic construct preferably targets any expressed protein to the secretory pathway of the host cell. An application of present invention is found in expressing the two chains of human insulin through two separate expression cassettes on the same methylotrophic yeast expression vector. Mature, bioactive human insulin molecules are secreted through this method without resorting to any post-translational cleavage process.