Milliporesigma
Senior Manager, Global Strategy
Merck Kgaa, Darmstadt, Germany
Associate, Global Graduate Program, Inhouse Consulting
Pbg Healthcare Consulting Aug 2016 - Apr 2017
Vice President of Alumni Relationships
Insite Oct 2015 - Mar 2017
Fellow
Pbg Healthcare Consulting Sep 2015 - Aug 2016
Director of Consulting
Education:
University of Pennsylvania 2012 - 2017
Doctorates, Doctor of Philosophy, Biology, Philosophy, Chemistry
Peking University 2008 - 2012
Bachelors, Bachelor of Arts, Bachelor of Science, Economics, Chemistry
Skills:
Molecular Biology Protein Expression Western Blotting Fluorescence Microscopy Cell Culture Biochemistry Fplc Fluorescence Molecular Cloning Protein Purification Immunoprecipitation Cell Biology Protein Chemistry Protein Engineering Confocal Microscopy Pcr Microscopy Polymerase Chain Reaction Immunohistochemistry Flow Cytometry Transfection Enzyme Kinetics
- Baltimore MD, US David Gracias - Baltimore MD, US Jayson Pagaduan - Windsor MD, US Anil Bhatta - Baltimore MD, US Xing Chen - Baltimore MD, US Qianru Jin - Cambridge MA, US
International Classification:
G01N 33/50 C12N 5/077 C12N 5/071 B01L 3/00
Abstract:
In one aspect, provided is a composition (biomimetic composition) that includes a biomimetic in vitro model of an arteriolar vessel comprising: at least one of 1) human smooth muscle cells and 2) human pulmonary endothelial cells; wherein the vessel recapitulates one or more of the overall tubular geometry, morphometrics, extracellular matrix constituents, cellular morphology, cellular alignment, and functional heterotypic connections between the human smooth muscle cells and/or the human endothelial cells as compared to an in vivo arteriolar vessel. A microfluidics-based model platform of the pulmonary circulation is provided. Methods of use include measuring flow in biomimetic vessels, and to determine the resistance of these biomimetic vessels in the setting of a variety of experimental conditions that recapitulate the pathobiology of pulmonary hypertension.