Skills:
Construct generation, molecular cloning of recombinogenic (unstable) inserts, transfection, DNA oligo purification, PCR, qRT-PCR, bisulphite PCR, cDNA cloning, 5' RACE, tdt mediated PCR, LM-PCR, shRNA cloning and protein knockdown validation, cis regulatory element prediction from microarray data, microRNA expression data prediction from microarray data, making competent bacterial cells, mammalian cell culture, mouse dissection, lentivirus and retrovirus production and collection, cell line generation, ZFN, CRISPR - Cas9, Southern blotting, northern blotting, western blotting, urea agarose heat denaturing gel for genomic Southerns, PCR southerns, microRNA northern and cloning, miRNA target validation, exonuclease assays, cell isolation by magnetic beads, FACS, novel assay conception and development (Hairpin-Capture of DNA End Structures), assay adaptation (urea agarose heat denaturing gel), mentoring, grant and proposal writing, grant and paper and proposal english language editing, DNA isolation, RNA isolation, molecular cloning, clone mutagenesis, next generation / high-throughput sequence library preparation (MiSeq and 454 sequencing platforms) and quality control on Agilent 2100 bionanalyzer and Qubit DNA quantiation, development of novel method for adaptor addition for next generation sequencing (PCR instead of ligation) and R statistical programming.