Yelena Freyzon from Chestnut Hill, MA, age 69

Yelena Freyzon Phones & Addresses

Chestnut Hill, MA
Arlington, MA
North Falmouth, MA
Brookline, MA
Cambridge, MA
Sharon, MA
20 Kensington Cir, Chestnut Hill, MA 02467

Work

Company:

Whitehead institute
Position:

Technical associate

Education

Degree:

MS in molecular biology
School / High School:

VNII Genetica: Institute of Microbial Genetics, Moscow, Russia
1980 to 1984
Specialities:

molecular and cell biology

Skills

Molecular Biology • Molecular Cloning • Dna Extraction • Pcr • Protein Chemistry • Dna • Transfection • Protein Expression • Protein Purification • Western Blotting • Immunohistochemistry • Cell Culture • Cancer • Cell Biology • Stem Cells • Fluorescence Microscopy • Biochemistry • Genetics • Microscopy • Sequencing • Life Sciences • Neuroscience • Cell Based Assays • Purification • Cell • Research • Microbiology • Science • Drug Discovery • Reverse Transcription Polymerase Chain R... • Sds Page • Rna Isolation • Gel Electrophoresis • Animal Models • Dna Sequencing

Languages

English • Russian

Industries

Biotechnology

Resumes

Chestnut Hill, Massachusetts

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Location:

20 Kensington Cir, Chestnut Hill, MA 02467

Industry:

Biotechnology

Work:

Whitehead Institute
Technical Associate

Education:

VNII Genetica: Institute of Microbial Genetics, Moscow, Russia 1980 - 1984
MS in molecular biology, molecular and cell biology

Skills:

Molecular Biology
Molecular Cloning
Dna Extraction
Pcr
Protein Chemistry
Dna
Transfection
Protein Expression
Protein Purification
Western Blotting
Immunohistochemistry
Cell Culture
Cancer
Cell Biology
Stem Cells
Fluorescence Microscopy
Biochemistry
Genetics
Microscopy
Sequencing
Life Sciences
Neuroscience
Cell Based Assays
Purification
Cell
Research
Microbiology
Science
Drug Discovery
Reverse Transcription Polymerase Chain Reaction
Sds Page
Rna Isolation
Gel Electrophoresis
Animal Models
Dna Sequencing

Languages:

English
Russian

Us Patents

Affinity Fluorescent Proteins And Uses Thereof
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US Patent:

20060035289, Feb 16, 2006
Filed:

Nov 1, 2004
Appl. No.:

10/978788
Inventors:

Paul Matsudaira - Wayland MA, US
Daniel Ehrlich - Lexington MA, US
Qiuhui Zhong - Lexington MA, US
Yelena Freyzon - Chestnut Hill MA, US
Assignee:

Whitehead Institute for Biomedical Research - CAMBRIDGE MA
International Classification:

G01N 33/567
C07K 14/435
US Classification:

435007210, 530350000
Abstract:

The present invention is related to an affinity fluorescent protein (aFP) comprising a modified fluorescent protein or molecule which comprises a heterologous amino acid sequence, thereby introducing a ligand-activated protein binding site, wherein the modified fluorescent protein displays an altered spectral property when the binding site is engaged with ligand relative to the spectral property displayed when the binding site is not engaged by ligand. The present invention also relates to an aFP expression cassette comprising a modified fluorescent protein nucleic acid sequence operatively linked to expression control sequences, wherein the modified fluorescent protein sequence comprises a recombinant peptide which comprises restriction endonuclease sites; and a host cell, comprising a recombinant nucleic acid molecule which comprises expression control sequences operatively linked to nucleotide sequence encoding an aFP, wherein said aFP comprises modified GFP molecule which comprises a mutated GFP molecule and a heterologous amino acid sequence which functions as a ligand-activated protein binding site, wherein the aFP an altered spectral property when the binding site is engaged with ligand relative to the spectral property displayed when the binding site is not engaged by ligand. The present invention also relates to a method of detecting the presence of a target ligand in a mixture of macromolecules. Also encompassed by the present invention is a method of a method of detecting the occurrence of a target ligand in a cell (e.g., a macrophage, a yeast cell).

Yelena Freyzon