Yi Zheng

US Patent:

6991911, Jan 31, 2006

Filed:

Dec 15, 2003

Appl. No.:

10/736005

Inventors:

Yi Feng Zheng - Wilmington DE, US

Assignee:

Dade Behring Inc. - Deerfield IL

International Classification:

G01N 33/53
G01N 33/531
C07K 16/00
C07K 17/02
C07D 317/58

US Classification:

435 79, 435961, 435975, 435 71, 435188, 436544, 436546, 5303889, 5303898, 530402, 530403, 530405, 4241751, 549444, 549443, 548526

Abstract:

Methods, compositions and kits are disclosed. The methods are directed to determining the presence of entactogen analytes such as, for example, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-methamphetamine (MDMA), 3,4-methylenedioxy-ethylamphetamine (MDEA) and 4-hydroxy-3-methoxy-methamphetamine (HMMA). The method comprises providing in combination in a medium (i) a sample suspected of containing the compound and (ii) an antibody raised against a compound of Formula I that comprises a protein. The medium is examined for the presence a complex comprising the compound and the antibody where the presence of such as complex indicates the presence of the compound in the sample. In one aspect of the above embodiment, the combination further comprises a label conjugate of the compound Formula I.

US Patent:

7022492, Apr 4, 2006

Filed:

Dec 15, 2003

Appl. No.:

10/736018

Inventors:

Yi Feng Zheng - Wilmington DE, US
Yali Yang - Wilmington DE, US

Assignee:

Dade Behring Inc. - Deerfield IL

International Classification:

G01N 33/53
G01N 33/531
C07K 16/00
C07K 17/02
C07D 317/58

US Classification:

435 79, 435961, 435975, 435 71, 435188, 436544, 436546, 5303889, 5303898, 530402, 530403, 530405, 4241751, 549444, 549443, 548526

Abstract:

Methods, compositions and kits are disclosed. Enzyme conjugates of Formula I are employed in assays for the determination of an methylenedioxyamphetamine, a methylene-dioxyethamphetamine, and/or a methylenedioxymethamphetamine. Immunogenic conjugates of Formula I are employed to prepare antibodies for an methylenedioxyamphetamine, a methylenedioxyethamphetamine, and/or for a methylene-dioxymethamphetamine for use in assays for the determination of an methylenedioxyamphetamine, a methylenedioxyethamphetamine, and/or a methylene-dioxymethamphetamine. The enzyme conjugates may also be employed to screen antibodies for use in such methods.

US Patent:

7037669, May 2, 2006

Filed:

Mar 22, 2004

Appl. No.:

10/805813

Inventors:

Yi Feng Zheng - Wilmington DE, US
Khaled A. Yamout - Carlsbad CA, US
Mae W. Hu - Los Altos Hills CA, US

Assignee:

Dade Behring Inc. - Deerfield IL

International Classification:

G01N 33/53
G01N 33/531
C07K 16/00
C07K 17/02
C07D 317/58

US Classification:

435 79, 435961, 435975, 435 71, 435188, 436544, 436546, 5303889, 5303898, 530402, 530403, 530405, 4241751, 549444, 549443, 548526, 564336

Abstract:

Methods, compositions and kits are disclosed. Enzyme conjugates of Formula I may be employed in assays for the determination of an amphetamine and/or a methamphetamine. Immunogenic conjugates of Formula I may be employed to prepare antibodies for an amphetamine and/or for a methamphetamine for use in assays for the determination of an amphetamine and/or a methamphetamine. The enzyme conjugates may also be employed to screen antibodies for use in such methods.

US Patent:

7115383, Oct 3, 2006

Filed:

Mar 22, 2004

Appl. No.:

10/806327

Inventors:

Yi Feng Zheng - Wilmington DE, US
Richard F. Parrish - San Jose CA, US
Johnny Valdez - Fremont CA, US

Assignee:

Dade Behring Inc. - Deerfield IL

International Classification:

G01N 33/535
G01N 33/531
C07K 16/00
C07K 17/02

US Classification:

435 79, 435961, 435975, 435 71, 435188, 436544, 436546, 5303889, 5303898, 530402, 530405

Abstract:

Methods, compositions and kits are disclosed. The compounds disclosed comprise an amphetamine moiety and a methamphetamine moiety linked together by a first linking group. A second linking group depends from the first linking group and comprises a functional group. The distance of the amphetamine moiety and the methamphetamine moiety from the point of linkage of the second linking group to the first linking group is approximately the same. The compounds may be linked to labels and used in assays for the detection of amphetamine and/or methamphetamine in samples suspected of containing these drugs.

US Patent:

7842475, Nov 30, 2010

Filed:

Jan 8, 2008

Appl. No.:

11/970658

Inventors:

Yi Feng Zheng - Wilmington DE, US
Tie Q. Wei - Bear DE, US

Assignee:

Siemens Healthcare Diagnostics Inc. - Deerfield IL

International Classification:

G01N 33/53

US Classification:

435 75, 435 71, 4352831, 4352872, 4352879, 436518, 436524, 436527

Abstract:

Reagents are disclosed for use in assays for analytes. The reagents are dry assay reagents that may be readily reconstituted for use in the assays. The dry assay reagents comprise a solid support and one or more molecules of a receptor immobilized on the solid support. The receptor comprises one or more binding sites for a ligand. A portion of a total number of the binding sites is bound to a conjugate comprising the ligand linked to a specific binding pair member and a portion of the total number of the binding sites is free. In use in an assay, a combination is provided in an aqueous medium comprising the sample and reagents for detecting the analyte wherein at least one of the reagents comprises the dry assay reagent mentioned above. The combination is incubated under conditions for binding of the analyte to one or more of the reagents. The presence and/or amount of binding of the analyte to one or more of the reagents is detected where the presence and/or amount of the binding is related to the presence and/or amount of the analyte in the sample.

US Patent:

8383424, Feb 26, 2013

Filed:

Apr 27, 2011

Appl. No.:

13/095506

Inventors:

Yi Feng Zheng - Wilmington DE, US
Deb K. Vickery - Landenberg PA, US
Susan C. Swann - Antioch IL, US

Assignee:

Siemens Healthcare Diagnostics - Tarrytown NY

International Classification:

G01N 33/546

US Classification:

436534, 514 11

Abstract:

Methods and reagents are disclosed for determining the presence and/or amount of cyclosporin A in a medium suspected of containing cyclosporin A. In the method a combination is provided in a medium. The combination comprises (i) the sample, (ii) a first member of a signal producing system (sps) associated with a first support wherein the first sps member is capable of activating a second member of the sps and wherein the first support is associated with a first member of a specific binding pair, and (iii) the second sps member associated with a second support wherein the second sps member is activatable by the first sps member. The second support comprises either (I) cyclosporin C or cyclosporin A and the combination further comprises a conjugate of an antibody for cyclosporin A and a second member of the specific binding pair or (II) antibody for cyclosporin A and the combination further comprises a conjugate of cyclosporin A and a second member of the specific binding pair. The combination is subjected to conditions for binding of cyclosporin A to the antibody for cyclosporin A. The first sps member is activated and the amount of signal generated by the second sps member is detected.

US Patent:

20050130243, Jun 16, 2005

Filed:

Dec 15, 2003

Appl. No.:

10/736004

Inventors:

Yi Zheng - Wilmington DE, US
Yali Yang - Wilmington DE, US

International Classification:

G01N033/53
G01N033/537
G01N033/543
C07K016/18
C07K014/47
C07D207/40

US Classification:

435007920, 530403000, 530388100, 548545000, 562442000

Abstract:

Methods, compositions and kits are disclosed. The methods are directed to determining the presence of entactogen analytes such as, for example, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-methamphetamine (MDMA), 3,4-methylenedioxy-ethylamphetamine (MDEA) and 4-hydroxy-3-methoxy-methamphetamine (HMMA). The method comprises providing in combination in a medium (i) a sample suspected of containing the compound and (ii) an antibody raised against a compound of Formula I that comprises a protein. The medium is examined for the presence a complex comprising the compound and the antibody where the presence of such as complex indicates the presence of the compound in the sample. In one aspect of the above embodiment, the combination further comprises a label conjugate of the compound Formula I.

US Patent:

20090170218, Jul 2, 2009

Filed:

Jan 2, 2008

Appl. No.:

11/968349

Inventors:

Yi Feng Zheng - Wilmington DE, US
Deb K. Vickery - Landenberg PA, US
Susan C. Swann - Antioch IL, US

Assignee:

DADE BEHRING INC. - Deerfield IL

International Classification:

G01N 33/566
C07D 259/00

US Classification:

436501, 540460

Abstract:

Methods and reagents are disclosed for determining the presence and/or amount of cyclosporin A in a medium suspected of containing cyclosporin A. In the method a combination is provided in a medium. The combination comprises (i) the sample, (ii) a first member of a signal producing system (sps) associated with a first support wherein the first sps member is capable of activating a second member of the sps and wherein the first support is associated with a first member of a specific binding pair, and (iii) the second sps member associated with a second support wherein the second sps member is activatable by the first sps member. The second support comprises either (I) cyclosporin C or cyclosporin A and the combination further comprises a conjugate of an antibody for cyclosporin A and a second member of the specific binding pair or (II) antibody for cyclosporin A and the combination further comprises a conjugate of cyclosporin A and a second member of the specific binding pair. The combination is subjected to conditions for binding of cyclosporin A to the antibody for cyclosporin A. The first sps member is activated and the amount of signal generated by the second sps member is detected. The amount of signal is related to the presence and/or amount of cyclosporin A in the sample.