Yong Sik Cho

US Patent:

42898536, Sep 15, 1981

Filed:

May 7, 1980

Appl. No.:

6/147353

Inventors:

James E. Bailey - Houston TX
Yong K. Cho - Rockford IL

Assignee:

Illinois Water Treatment Company - Rockford IL

International Classification:

C12N 1102

US Classification:

435177

Abstract:

Enzymes are immobilized on activated carbon supports at high load levels and with high stability by reacting an activated carbon support with a carbodiimide or an isoxazolium salt to form active organic radicals on the surface of the activated carbon support and thereafter treating the carbon support with an enzyme solution to immobilize the enzyme on the carbon support. The immobilization of enzymes on activated carbon provides immobilized enzymes which are easily handled and which are stabilized against denaturation by hydrogen peroxide.

US Patent:

42040418, May 20, 1980

Filed:

Oct 3, 1977

Appl. No.:

5/838544

Inventors:

James E. Bailey - Houston TX
Yong K. Cho - Rockford IL

Assignee:

Illinois Water Treatment Company - Rockford IL

International Classification:

C07G 702

US Classification:

435177

Abstract:

Enzymes are immobilized on activated carbon supports at high load levels and with high stability by a procedure whereby a carbon support is first activated with a water-soluble carbodiimide derivative which forms a highly reactive intermediate with carboxyl and other active organic radicals on the surface of the carbon support; thereafter the complex of the carbon and carbodiimide is treated with an enzyme solution whereby the enzyme displaces the carbodiimide and forms a carbon-enzyme complex wherein the enzyme is immobilized and yet the carbon retains its surface activity. Preferred immobilizing agents are quaternary ammonium forms of aminocarbodiimides or hydrochloric acid salts of water-soluble carbodiimides. The immobilization of the enzymes on activated carbon provides materials which are easily handled and which are stabilized against denaturation by hydrogen peroxide.