Jan 2014 to 2000 Graduate AssistantReckitt Benckiser Pharmaceuticals Inc. Richmond, VA Apr 2013 to Jan 2014 Clinical Pharmacology ScientistUniversity of Akron Akron, OH Aug 2008 to Dec 2012 Graduate Student Researcher/Lab Assistant/Fish caregiverUniversity of Akron Akron, OH Jan 2009 to May 2010 Graduate Teaching AssistantUniversity of Akron Akron, OH Sep 2009 to Nov 2009 Research and Lab AssistantInstitution of Material Medical, Chinese Academy of Medical Sciences and Peking Union Medical Collage
Jul 2007 to May 2008 Internship and research assistant
Education:
Virginia Commonwealth University Richmond, VA 2014 to 2015 MS in Statistic sciencesUniversity of Akron Akron, OH 2008 to 2012 Doctor of Philosophy in Integrated BioscienceChina Pharmaceutical University Nanjing, CN 2004 to 2008 Bachelor of Science in Pharmacology
Skills:
Molecular biology techniques: Design, synthesis and microinjection of antisense morpholino oligonucleotide (MO); RNA and DNA isolation; cDNA synthesis and library construction and screening; PCR, primer construction and cloning; Immunohistochemistry for both whole mount embryo and tissue sections Whole mount in situ hybridization. Proteomics techniques: Protein extraction and purification Two-dimensional electrophoresis; HPLC; Mass spectrometry; Western blotting. Computer techniques: Two-dimensional gel analyzed software (SPOT Basic; Melanie); Online databases searching and sequencing alignment (Blast2Go); Biostatistics (StatCrunch); MS Word, Excel, PowerPoint; Adobe Photoshop (basic level) SQL (basic level) Clinical approach: Parenteral injections; Observation of focal lesion; Tissue isolation; Animal handling; PK/PD.
Reference: APOE3ch alters microglial response and suppresses A-induced tau seeding and spread by Yun Chen, Sihui Song, Samira Parhizkar, Jennifer Lord, Yiyang Zhu, Michael R. Strickland, Chanung Wang, Jiyu Park, G. Travis Tabor, Hong Jiang, Kevin Li, Albert A. Davis, Carla M. Yuede, Marco Colonna
Date: Dec 25, 2023
Category: Health
Source: Google
Clues to preventing Alzheimer's come from patient who evaded disease, despite genetics
To solve this puzzle, Holtzman and first author Yun Chen, a graduate student, turned to genetically modified mice. They took mice genetically predisposed to overproduce amyloid and modified them to carry the human APOE gene with the Christchurch mutation. Then, they injected a tiny bit of human tau