Yvonne Raysor Reid

US Patent:

20090155232, Jun 18, 2009

Filed:

Jul 14, 2008

Appl. No.:

12/218301

Inventors:

Jaya Gaddipati - Boyds MD, US
Judith Kantor - Rockville MD, US
Yvonne A. Reid - Haymarket VA, US

Assignee:

American Type Culture Collection - Manassas VA

International Classification:

A61K 48/00
C12N 5/10
C12N 15/85
C12Q 1/02

US Classification:

424 9321, 435370, 435455, 435 29

Abstract:

The present invention relates to the discovery of immortalized neonatal human hepatocytes that exhibit phenotypic features of human hepatic progenitor cells. The invention is also directed to a method of obtaining telomerase-immortalized neonatal human hepatocytes that exhibit phenotypic features of human hepatic progenitor cells. Furthermore, the instant invention describes methods of using the immortalized neonatal human hepatocytes in cellular therapies, toxicological studies, pharmacokinetic studies, metabolic studies, therapeutic gene delivery and for the production of fully differentiated hepatocytes.

US Patent:

20080113349, May 15, 2008

Filed:

Nov 3, 2006

Appl. No.:

11/592117

Inventors:

Pranvera Ikonomi - Springfield VA, US
Jason Cooper - Fairfax VA, US
Greg Sykes - Fairfax VA, US
Yvonne Reid - Haymarket VA, US

International Classification:

C12Q 1/68
C07H 21/00
C12Q 1/00

US Classification:

435 6, 435 4, 536 2433

Abstract:

The present invention relates to a PCR (polymerase chain reaction) based assay that is useful for detecting, identifying, quantitating and analysis of a target nucleic acid (target nucleic acid hereinafter) in a sample. More specifically, the present invention relates to a PCR based assay that can improve accuracy in detecting, identifying, and quantitating contamination in mammalian cell lines using a PCR-based assay of nucleic acid oligonucleotides (oligoprobes) having a sequence expected to be complementary to a target nucleic acid sequence in a sample. More specifically, the sample may contain either cells or nucleic acid isolated from a cell line. The present invention also relates to a detection kit using the PCR-based assay. The invention also involves a method for using specifically produced nucleic acids complementary to specific sequences or populations of different sequences of the cytochrome c oxidase I (COI) and/or cytochrome b, to detect, identify, and quantify specific organisms, groups of organisms, groups of eukaryotic cells or viruses in cells.