Zhi H Lin

US Patent:

20200140388, May 7, 2020

Filed:

Jun 6, 2018

Appl. No.:

16/620372

Inventors:

- Cambridge MA, US
Jinxu Gao - Somerville MA, US
Yuka Amako - Watertown MA, US
Chia Fu Chang - Cambridge MA, US
Zhi Lin - Cambridge MA, US
Hung-Yi Wu - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C07D 229/02
C07D 401/12
G01N 33/68
C07D 487/14

Abstract:

Provided herein are methods, systems, kits, and compositions useful for determining small molecule-protein interactions and protein-protein interactions. The photo-click tags provided herein can be conjugated to a small molecule or amino acid analog to provide compounds that can be integrated into a protein through photo-conjugation, allowing for identification of a small molecule-protein interaction or protein-protein interaction to elucidate the small molecules mechanism of action or the protein targeted by the small molecule. In some embodiments, the photo-click tags comprise a photo-conjugation moiety and a click chemistry handle, allowing for the attachment of various functional groups (e.g., affinity tags) to the small molecule or amino acid analog.