Zissimos Mourelatos

US Patent:

8532937, Sep 10, 2013

Filed:

Feb 22, 2012

Appl. No.:

13/402646

Inventors:

Artemis G. Hatzigeorgiou - Philadelphlia PA, US
Zissimos Mourelatos - Philadelphia PA, US

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C07H 21/02

US Classification:

702 20, 536 245

Abstract:

Method of identifying a microRNA-recognition element and of generating microRNAs are disclosed. System and computer programs for performing such methods are disclosed. Recombinant nucleic acid molecule comprising a heterologous coding sequences and one or more MREs are also disclosed as are isolated nucleic acid molecule comprising one or more MRE sequences and being free of a coding sequence operably linked to regulatory elements. MicroRNA generated by a methods of the invention and the use of the microRNAs to downregulate gene expression are disclosed.

US Patent:

20060078925, Apr 13, 2006

Filed:

Sep 22, 2005

Appl. No.:

11/232820

Inventors:

Zissimos Mourelatos - Philadelphia PA, US
Peter Nelson - Philadelphia PA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

Provided are DNA microarray techniques that allow hybridization without RNA amplification, without using cDNA, and without labeling the nucleic acid prior to hybridization. Referred to as the Double-stranded Exonuclease Protection (DEP) assay, the technique permits the sample RNA to be used directly for hybridization, without manipulation in any way. Further provided is a microarray technique for high-throughput miRNA gene expression analyses, termed the RNA-primed, Array-based, Klenow Enzyme (RAKE) assay. The RAKE assay is a sensitive and specific technique for assessing single-stranded DNA and RNA targets, and offers specific advantages over Northern blots.

US Patent:

20070026403, Feb 1, 2007

Filed:

Jul 15, 2004

Appl. No.:

10/564707

Inventors:

Artemis Hatzigeorgiou - Philadelphia PA, US
Zissimos Mourelatos - Philadelphia PA, US

International Classification:

C12Q 1/68
G06F 19/00
C12P 19/34

US Classification:

435006000, 435091200, 702020000

Abstract:

Method of identifying a microRNA-recognition element and of generating microRNAs are disclosed. System and computer programs for performing such methods are disclosed. Recombinant nucleic acid molecule comprising a heterologous coding sequences and one or more MREs are also disclosed as are isolated nucleic acid molecule comprising one or more MRE sequences and being free of a coding sequence operably linked to regulatory elements. MicroRNA generated by a methods of the invention and the use of the microRNAs to downregulate gene expression are disclosed.

US Patent:

20090012016, Jan 8, 2009

Filed:

Oct 22, 2004

Appl. No.:

10/576481

Inventors:

Zissimos Mourelatos - Philadelphia PA, US
Anup Sharma - Philadelphia PA, US
Marianthi Kiriakidou - Philadelphia PA, US

International Classification:

A61K 31/7105
C40B 30/02
A61P 43/00
C07H 21/02

US Classification:

514 44, 506 8, 536 245

Abstract:

Methods of identifying, designing and synthesizing uniquely targeting siRNA nucleotide sequences for a target mRNA sequence of a target species are disclosed. Methods of identifying, designing and synthesizing miRNA nucleotide sequences that does not function as a siRNA nucleotide sequence for mRNA of a target species are disclosed. Method of inhibiting expression of a target mRNA molecule are disclosed sequence. siRNA molecules including uniquely targeting siRNA molecules and RISCs comprising the same are disclosed. miRNA molecules that do not function as siRNA nucleotide sequences for mRNA of a target species are disclosed.

US Patent:

20140106351, Apr 17, 2014

Filed:

Sep 9, 2013

Appl. No.:

14/022008

Inventors:

Artemis G. Hatzigeorgiou - Athens, GR
Zissimos Mourelatos - Philadelphia PA, US

Assignee:

The Trustees Of The University of Pennsylvania - Philadelphia PA

International Classification:

C12N 15/113
G01N 21/76

US Classification:

435 611, 536 231, 536 245, 435375, 4353201

Abstract:

Method of identifying a microRNA-recognition element and of generating microRNAs are disclosed. System and computer programs for performing such methods are disclosed. Recombinant nucleic acid molecule comprising a heterologous coding sequences and one or more MREs are also disclosed as are isolated nucleic acid molecule comprising one or more MRE sequences and being free of a coding sequence operably linked to regulatory elements. MicroRNA generated by a methods of the invention and the use of the microRNAs to downregulate gene expression are disclosed.