The technology described herein is directed to methods of determining oligonucleotide sequences, e.g. by enriching target sequences prior to sequencing the sequences.
Methods For Determining A Nucleotide Sequence Contiguous To A Known Target Nucleotide Sequence
- Boston MA, US Long Phi LE - Boston MA, US Zongli ZHENG - Boston MA, US
Assignee:
THE GENERAL HOSPITAL CORPORATION - Boston MA
International Classification:
C12Q 1/6855 C12Q 1/6874 C12Q 1/6806
Abstract:
The technology described herein is directed to methods of determining oligonucleotide sequences, e.g. by enriching target sequences prior to sequencing the sequences.
- Boston MA, US - Boulder CO, US Zongli ZHENG - Boston MA, US Jason MYERS - Golden CO, US Joshua STAHL - Boulder CO, US
Assignee:
The General Hospital Corporation - Boston MA ArcherDX, Inc. - Boulder CO
International Classification:
C12Q 1/6806 C12Q 1/6809
Abstract:
Aspects of the technology disclosed herein relate to methods for preparing and analyzing nucleic acids. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.
- Boston MA, US Long Phi Le - Boston MA, US Zongli Zheng - Boston MA, US
Assignee:
The General Hospital Corporation - Boston MA
International Classification:
C12Q 1/686
Abstract:
Aspects of the technology disclosed herein relate to methods for preparing and analyzing nucleic acids. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.
Methods For Determining A Nucleotide Sequence Contiguous To A Known Target Nucleotide Sequence
- Boston MA, US Long Phi Le - Boston MA, US Zongli Zheng - Boston MA, US
Assignee:
THE GENERAL HOSPITAL CORPORATION - Boston MA
International Classification:
C12Q 1/68
Abstract:
The technology described herein is directed to methods of determining oligonucleotide sequences, e.g. by enriching target sequences prior to sequencing the sequences.
Methods And Systems For Droplet Tagging And Amplification
- Cambridge MA, US - Boston MA, US Zongli Zheng - Boston MA, US Huidan Zhang - Cambridge MA, US
International Classification:
C12N 15/10
Abstract:
The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfiuidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid “tags,” and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.
- Boston MA, US - Boulder CO, US Zongli Zheng - Boston MA, US Jason Myers - Golden CO, US Joshua Stahl - Boulder CO, US
International Classification:
C12Q 1/68
Abstract:
Aspects of the technology disclosed herein relate to methods for preparing and analyzing nucleic acids. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.
- Boston MA, US Long Phi Le - Boston MA, US Zongli Zheng - Boston MA, US
International Classification:
C12Q 1/68
Abstract:
Aspects of the technology disclosed herein relate to methods for preparing and analyzing nucleic acids. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.