Ben J Johnson

US Patent:

6372106, Apr 16, 2002

Filed:

Jul 26, 1999

Appl. No.:

09/361485

Inventors:

Ben F. Johnson - Palo Alto CA
Eric S. Nordman - Palo Alto CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N 2726

US Classification:

204455, 204451, 204601, 204605

Abstract:

Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.

US Patent:

7169276, Jan 30, 2007

Filed:

Feb 13, 2002

Appl. No.:

10/075404

Inventors:

Ben F. Johnson - Palo Alto CA, US
Eric S. Nordman - Palo Alto CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

G01N 27/447

US Classification:

204451, 204455

Abstract:

Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described. Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.

US Patent:

20060160122, Jul 20, 2006

Filed:

Feb 15, 2006

Appl. No.:

11/355872

Inventors:

Michael Harrold - San Mateo CA, US
Aldrich Lau - Palo Alto CA, US
Ben Johnson - Palo Alto CA, US
Gilbert Amparo - Hayward CA, US
Frank Mercer - Belmont CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.

US Patent:

20100196912, Aug 5, 2010

Filed:

Apr 9, 2010

Appl. No.:

12/757888

Inventors:

Michael P. Harrold - San Mateo CA, US
Aldrich N.K. Lau - Palo Alto CA, US
Ben F. Johnson - Palo Alto CA, US
Gilbert P. Amparo - Hayward CA, US
Frank W. Mercer - Belmont CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C07H 1/06
C09K 3/00
G01N 27/26

US Classification:

435 6, 536 254, 252184, 204451

Abstract:

A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.

US Patent:

20120305838, Dec 6, 2012

Filed:

Jun 5, 2012

Appl. No.:

13/489381

Inventors:

Michael P. Harrold - San Mateo CA, US
Aldrich N.K. Lau - Palo Alto CA, US
Ben F. Johnson - Palo Alto CA, US
Gilbert P. Amparo - Hayward CA, US
Frank W. Mercer - Belmont CA, US

Assignee:

APPLIED BIOSYSTEMS, LLC - Carlsbad CA

International Classification:

B01J 39/18
B01J 39/20
B01J 41/14
B01J 41/12

US Classification:

252184, 521 28

Abstract:

A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.

US Patent:

58913130, Apr 6, 1999

Filed:

Nov 8, 1995

Appl. No.:

8/554247

Inventors:

Ben F. Johnson - Palo Alto CA
Steven M. Menchen - Fremont CA
Will Bloch - San Mateo CA

Assignee:

The Perkin-Elmer Corp. - Foster City CA

International Classification:

C25B 700

US Classification:

204451

Abstract:

A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20K Daltons. Furthermore, the invention includes stable denaturants useful for the electrophoresis of nucleic acids.

US Patent:

54683653, Nov 21, 1995

Filed:

Sep 22, 1993

Appl. No.:

8/125623

Inventors:

Steven M. Menchen - Fremont CA
Mitchell A. Winnik - Toronto, CA
Ben F. Johnson - Palo Alto CA

Assignee:

Applied Biosystems, Inc. - Foster City CA

International Classification:

C25B 700

US Classification:

204299R

Abstract:

A viscous electrophoresis separation medium is disclosed. The medium is formed by a matrix formed by a copolymer composed of hydrophilic polymer segments having selected, substantially uniform segment lengths, and a plurality of hydrophobic polymer segments carried on, and spaced from one another by the hydrophilic polymer segments. Also disclosed is an electrophoresis method which employs the separation medium, and novel copolymers used in forming the medium.

US Patent:

60516364, Apr 18, 2000

Filed:

Mar 9, 1999

Appl. No.:

9/264757

Inventors:

Ben F. Johnson - Palo Alto CA
Steven M. Menchen - Fremont CA
Will Bloch - San Mateo CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C08K 5151
C08K 53415

US Classification:

524 98

Abstract:

A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20K Daltons. Furthermore, the invention includes stable denaturants useful for the electrophoresis of nucleic acids.