Skills:
Radioligand binding, Enzyme activity assays, Scintillation proximity assay to measure G-protein subtype specific activation, [35S] GTPS, [3H] cAMP, [3H] noradrenaline, TUNEL functional assays, Osteoblast differentiation and growth assays, Fluorescence polarization to measure ligand binding, Fluorescent Ca2+ measurement, FRET (sensitized & photobleaching using fluorescent widefield/confocal microscopy), Confocal microscopy, RNA extraction and qPCR, PCR (mutagenesis and various techniques), Cloning (traditional, Goldengate, Gibson assembly, recombinase), Generation of adenoviral particles for transfection, Western blot, Immunoprecipitation, Culture of mammalian cells, Generation of stable cell lines, transgenic mouse, Isolation and culture of PBMCs from mouse femurs, Extraction of cardiac myocytes from rat hearts, Transfection (chemical, electroporation, viral), Bioinformatics - Intermediate level: protein modeling, molecular dynamics, molecular biology, Microsoft Excel, Powerpoint etc. Graphpad Prism, ImageJ, Programming experience in Basic, script writing, Excellent organization skills: planning and running multiple experiments, ability to prioritize tasks and set and meet deadlines, Positive interpersonal skills as part of research teams and teaching students, Excellent oral presentation skills, Scientific report/grant writing.