Skills:
Molecular biology skills<br/> PCR, PCR-based gene synthesis, cloning, DNA isolation, Gibson assembly, yeast strain construction<br/> Vector NTI and DNASTAR (Lasergene)<br/><br/>Metabolic engineering skills<br/> Expression level control via codon optimization, engineering of diverse promoters and terminators, markers, plasmid stability, gene copy number control, manipulation of activators or repressors, protease level control, manipulation of protein ubiquitination, and culture media optimization.<br/> Computational method: OptKnock<br/><br/>Protein engineering skills<br/> Mutagenesis<br/> Purifications: His-tag purification, size exclusion, salt precipitation, avidin affinity chromatography, hydroxyapatite chromatography<br/> Protein gel, Western blot, Enzyme assays (Bradford assay, NADPH assay, fluorescence assay, xylitol assay)<br/><br/>Bioinformatics tools<br/> Primer design, databases (NCBI, UniProt, ExPASy, EcoCyc, SGD), Blast, sequence alignment (DNA or Protein), sequencing data analysis<br/><br/>Analytical tools<br/> HPLC (metabolite and carbohydrate analysis), LC-MS, qPCR, RT-PCR, UV-Vis, Fluorescence spectroscopy, Microscopy, Plate reader