Skills:
INTERPERSONAL AND COMMUNICATION SKILL SET: a positive attitude, an empathetic nature, and the ability to build rapport and trust among my colleagues. Speaking clearly, succinctly and in an open and non-confrontational manner. Possessing a team player mentality, where success of the team is the primary goal and is accomplished when team members work together, have a willingness to share knowledge, take the initiative to tackle difficult problems and understand team dynamics. IMMUNOLOGY SKILL SET: 10-color flow cytometry analysis, lymphocyte, goblet cell and dendritic cell isolation from mouse intestine, FACS sorting and in vitro cell culture of sorted cells, ELISA, ELISPOT, qRT-PCR, 4-color fluorescence microscopy, histology, mouse husbandry and genotyping, software including FlowJo, GraphPad Prism and Adobe Illustrator. CELL AND MOLECULAR BIOLOGY SKILL SET: Tissue culture, bacteria cell culture, cytotoxicity and cell viability via MTT assay, endotoxin testing via LAL assay, subcloning, site directed mutagenesis, plasmid propagation, cell transfection, immunoblot, PCR, immunocytochemistry, in vitro kinase activity, immunoprecipitation, mammalian cell fractionation, 3H-Thymidine incorporation.