Sung Y Lee

US Patent:

20130203140, Aug 8, 2013

Filed:

Aug 3, 2012

Appl. No.:

13/566923

Inventors:

Martien H. BERGSMA - Zoetermeer, NL
Anthony R. Calabria - Wilmington DE, US
Gopal K. Chotani - Cupertino CA, US
William A. Cuevas - San Francisco CA, US
Gang Duan - Shanghai, CN
Sung Ho Lee - North Liberty IA, US
Ying Qian - Wuxi City, CN
Vivek Sharma - North Liberty IA, US
Jayarama K. Shetty - Pleasanton CA, US
Bruce A. Strohm - Beloit WI, US
Paula Johanna Maria Teunissen - Saratoga CA, US
Hongxian Xu - Wuxi City, CN

Assignee:

Danisco US Inc. - Palo Alto CA

International Classification:

C12P 5/00

US Classification:

435157, 435166, 435167, 435155, 4352891

Abstract:

Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

US Patent:

20210047664, Feb 18, 2021

Filed:

Aug 25, 2020

Appl. No.:

17/002091

Inventors:

- Palo Alto CA, US
Ling Hua - Hockessin DE, US
Sung Ho Lee - Palo Alto CA, US
Jalsen Li - Palo Alto CA, US
Jayarama K. Shetty - Palo Alto CA, US
Zhongmei Tang - Shanghai, CN
Bo Zhang - Shanghai, CN
Kun Zhong - Shanghai, CN

International Classification:

C12P 19/14
C12N 9/34
C12P 19/02

Abstract:

Fungal glucoamylases from —expressed in host cells (AfGATR) are provided. host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA . AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by glucoamylase (AnGA) or native AfGA expressed in . AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

US Patent:

20190177757, Jun 13, 2019

Filed:

Feb 21, 2019

Appl. No.:

16/281989

Inventors:

- Palo Alto CA, US
Ling Hua - Hockessin DE, US
Sung Ho Lee - Palo Alto CA, US
Jalsen Li - Palo Alto CA, US
Jayarama K Shetty - Palo Alto CA, US
Zhongmei Tang - Shanghai, CN
Bo Zhang - Shanghai, CN
Kun Zhong - Shanghai, CN

International Classification:

C12P 19/14
C12P 19/02
C12N 9/34

Abstract:

Fungal glucoamylases from —expressed in host cells (AfGATR) are provided. host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA . AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by glucoamylase (AnGA) or native AfGA expressed in . AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

US Patent:

20160115509, Apr 28, 2016

Filed:

Nov 21, 2013

Appl. No.:

14/649167

Inventors:

- Palo Alto CA, US
Ling Hua - Hockessin DE, US
Sung Ho Lee - North Liberty IA, US
Jalsen Li - Shanghai, CN
Jayarama K. Shetty - Pleasanton CA, US
Zhongmei Tang - Shanghai, CN
Bo Zhang - Shanghai, CN
Kun Zhong - Shanghai, CN

International Classification:

C12P 19/14
C12P 19/02
C12N 9/34

Abstract:

Fungal glucoamylases from —expressed in host cells (AfGATR) are provided. host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA . AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by glucoamylase (AnGA) or native AfGA expressed in . AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

US Patent:

20160108448, Apr 21, 2016

Filed:

Nov 21, 2013

Appl. No.:

14/649168

Inventors:

- Palo Alto CA, US
Ling Hua - Hockessin DE, US
Sung Ho Lee - North Liberty IA, US
Jayarama K. Shetty - Pleasanton CA, US
Bruce A. Strohm - Beloit WI, US
Zhongmei Tang - Shanghai, CN
Donald E. Ward - Cedar Rapids IA, US

International Classification:

C12P 19/16
C12P 19/02

Abstract:

Fungal glucoamylases from that are expressed in host cells (AfGATR) are provided. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a simultaneous liquefaction and saccharification process in the presence of alpha amylase, such as alpha-amylase (AkAA). This greatly reduces the combined run time of liquefaction and saccharification reaction, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase.