Aaron Dean Brooks

US Patent:

20220246235, Aug 4, 2022

Filed:

Apr 21, 2022

Appl. No.:

17/726250

Inventors:

- Boulder CO, US
Joshua SHORENSTEIN - Boulder CO, US
Andrew GARST - Boulder CO, US
Craig STRUBLE - Pleasanton CA, US
Miles GANDER - Boulder CO, US
Juhan KIM - Pleasanton CA, US
Bryan LELAND - Boulder CO, US
Eileen SPINDLER - Boulder CO, US
Paul HARDENBOL - Pleasanton CA, US
Aaron BROOKS - Albuquerque NM, US
Eric ABBATE - Boulder CO, US

International Classification:

G16B 20/30
G16B 40/20
C12N 15/10
G06N 3/08

Abstract:

The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.