Dr. Liu graduated from the Temple University School of Medicine in 1997. He works in Philadelphia, PA and specializes in Internal Medicine. Dr. Liu is affiliated with Hospital Of The University Of Pennsylvania and Mercy Philadelphia Hospital.
- San Diego CA, US - Cambridge, GB Shaoping Lu - Palo Alto CA, US Simon Prince - Carlsbad CA, US Merek C. Siu - Alameda CA, US Stanley S. Hong - Palo Alto CA, US Aaron Liu - Riverside CA, US Gary Mark Skinner - Kedington, GB Geraint Wyn Evans - Cambridge, GB
The disclosure provides for structured illumination microscopy (SIM) imaging systems. In one set of implementations, a SIM imaging system may be implemented as a multi-arm SIM imaging system, whereby each arm of the system includes a light emitter and a beam splitter (e.g., a transmissive diffraction grating) having a specific, fixed orientation with respect to the system's optical axis. In a second set of implementations, a SIM imaging system may be implemented as a multiple beam splitter slide SIM imaging system, where one linear motion stage is mounted with multiple beam splitters having a corresponding, fixed orientation with respect to the system's optical axis. In a third set of implementations, a SIM imaging system may be implemented as a pattern angle spatial selection SIM imaging system, whereby a fixed two-dimensional diffraction grating is used in combination with a spatial filter wheel to project one-dimensional fringe patterns on a sample.
Illumination For Fluorescence Imaging Using Objective Lens
- San Diego CA, US Stanley S. Hong - Palo Alto CA, US Frederick Erie - Carlsbad CA, US Alex Nemiroski - San Diego CA, US M. Shane Bowen - Encinitas CA, US Danilo Condello - San Francisco CA, US Dietrich Dehlinger - San Francisco CA, US Marco A. Krumbuegel - San Ramon CA, US Anthony Lam - Fremont CA, US Aaron Liu - Irvine CA, US Bojan Obradovic - Carlsbad CA, US Mark Pratt - Belmont CA, US
International Classification:
G01N 21/64
Abstract:
A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.
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