Amy C Hsu

US Patent:

6358716, Mar 19, 2002

Filed:

May 11, 1999

Appl. No.:

09/297928

Inventors:

Ben A. Bulthuis - Leiden, NL
Anthony Arthur Gatenby - Wilmington DE
Sharon Loretta Haynie - Philadelphia PA
Amy Kuang-Hua Hsu - Redwood City CA
Richard D. Lareau - Mountain View CA

Assignee:

E. I. du Ponte de Nemours and Company - Wilmington DE

International Classification:

C12P 720

US Classification:

435159

Abstract:

Recombinant organisms are provided comprising genes encoding a glycerol-3-phosphate dehydrogenase and/or a glycerol-3-phosphatase activity useful for the production of glycerol from a variety of carbon substrates.

US Patent:

20040235099, Nov 25, 2004

Filed:

Dec 18, 2003

Appl. No.:

10/739542

Inventors:

Mark Payne - Wilmington DE, US
Stephen Picataggio - Landenbert PA, US
Amy Hsu - Redwood City CA, US
Ramesh Nair - Cupertino CA, US
Fernando Valle - Burlingam CA, US
Philippe Soucaille - Deyme, FR
Donald Trimbur - Landenberg PA, US

International Classification:

C12N009/92
C07H021/04
C12N001/21

US Classification:

435/069100, 435/234000, 435/252300, 435/320100, 536/023200

Abstract:

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

US Patent:

20070065867, Mar 22, 2007

Filed:

Oct 2, 2006

Appl. No.:

11/541810

Inventors:

Mark Payne - Wilmington DE, US
Stephen Picataggio - Landenberg PA, US
Amy Hsu - Redwood City CA, US
Ramesh Nair - Cupertino CA, US
Fernado Valle - Burlingame CA, US
Philippe Soucaille - San Francisco CA, US
Donald Trimbur - Redwood City CA, US

International Classification:

C40B 30/06
C40B 40/10
C07H 21/04
C12N 15/74

US Classification:

435006000, 536023200, 435252350, 435471000

Abstract:

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

US Patent:

20090011463, Jan 8, 2009

Filed:

Apr 7, 2005

Appl. No.:

10/591852

Inventors:

Walter Weyler - San Francisco CA, US
Amy Kuang-Hua Hsu - Redwood City CA, US

International Classification:

C12P 21/04
C12N 1/20
C12N 9/56
C12N 15/74
C12N 15/11
C12N 15/00

US Classification:

435 694, 4352523, 43525231, 435222, 536 232, 4353201, 43525233, 435 691, 435 696, 435471

Abstract:

The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins, wherein the pckA gene has been modified or deleted. In particular, the present invention relates to Gram-positive microorganisms, such as species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been modified and/or inactivated (e.g., pckA), and preferably wherein one or more chromosomal genes (e.g., pckA) have been modified and/or deleted from the chromosome. In some further embodiments, one or more indigenous chromosomal regions have been modified and/or deleted from a corresponding wild-type host chromosome.

US Patent:

20090156430, Jun 18, 2009

Filed:

Feb 13, 2009

Appl. No.:

12/370864

Inventors:

Mark S. Payne - Wilmington DE, US
Stephen K. Picataggio - Gaithersburg MD, US
Amy Kuang-Hua Hsu - Redwood City CA, US
Ramesh V. Nair - Cupertino CA, US
Fernando Valle - Burlingame CA, US
Philippe Soucaille - Deyme, FR
Donald E. Trimbur - Palo Alto CA, US

Assignee:

E. I. DU PONT DE NEMOURS AND COMPANY - Wilmington DE

International Classification:

C40B 40/08
C12N 15/11
C12N 15/00

US Classification:

506 17, 536 241, 4353201

Abstract:

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

US Patent:

20110046014, Feb 24, 2011

Filed:

Nov 5, 2010

Appl. No.:

12/940225

Inventors:

MARK S. PAYNE - Wilmington DE, US
Stephen K. Picataggio - Solana Beach CA, US
Amy Kuang-Hua Hsu - Redwood City CA, US
Ramesh V. Nair - Cupertino CA, US
Fernando Valle - Burlingame CA, US
Philippe Soucaille - Deyme, FR
Donald E. Trimbur - Palo Alto CA, US

Assignee:

E. I. DU PONT DE NEMOURS AND COMPANY - Wilmington DE

International Classification:

C40B 40/08
C07H 21/00
C12N 15/63
C07H 21/04
C12N 1/21

US Classification:

506 17, 536 232, 4353201, 536 241, 43525233

Abstract:

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

US Patent:

60134941, Jan 11, 2000

Filed:

Nov 12, 1997

Appl. No.:

8/968563

Inventors:

Charles E. Nakamura - Claymont DE
Anthony A. Gatenby - Wilmington DE
Amy Kuang-Hua Hsu - Redwood City CA
Richard D. La Reau - Mountain View CA
Sharon L. Haynie - Philadelphia PA
Maria Diaz-Torres - San Mateo CA
Donald E. Trimbur - Redwood City CA
Gregory M. Whited - Belmont CA
Vasantha Nagarajan - Wilmington DE
Mark S. Payne - Wilmington DE
Stephen K. Picataggio - Landenberg PA
Ramesh V. Nair - Wilmington DE

Assignee:

E. I. du Pont de Nemours and Company - Wilmington DE
Genencor International - Palo Alto CA

International Classification:

C12P 718
C12N 119
C12N 121
C12N 1531

US Classification:

435158

Abstract:

Recombinant organisms are provided comprising genes encoding glycerol-3-phosphate dehydrogenase, glycerol-3-phosphatase, glycerol dehydratase and 1,3-propanediol oxidoreductase activites useful for the production of 1,3-propanediol from a variety of carbon substrates.