Cathleen A Zimmerman

US Patent:

7634125, Dec 15, 2009

Filed:

Aug 20, 2008

Appl. No.:

12/194842

Inventors:

William E. Ortyn - Bainbridge Island WA, US
David A. Basiji - Seattle WA, US
Philip Morrissey - Bellevue WA, US
Thaddeus George - Seattle WA, US
Brian Hall - Seattle WA, US
Cathleen Zimmerman - Bainbridge Island WA, US
David Perry - Woodinville WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133, 356326

Abstract:

Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.

US Patent:

7634126, Dec 15, 2009

Filed:

Jan 29, 2009

Appl. No.:

12/362170

Inventors:

William E. Ortyn - Bainbridge Island WA, US
David A. Basiji - Seattle WA, US
Philip Morrissey - Bellevue WA, US
Thaddeus George - Seattle WA, US
Brian Hall - Seattle WA, US
Cathleen Zimmerman - Bainbridge Island WA, US
David Perry - Woodinville WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133, 356326

Abstract:

Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.

US Patent:

7925069, Apr 12, 2011

Filed:

Sep 29, 2009

Appl. No.:

12/569612

Inventors:

William E. Ortyn - Bainbridge Island WA, US
David A. Basiji - Seattle WA, US
Philip Morrissey - Bellevue WA, US
Thaddeus George - Seattle WA, US
Brian Hall - Seattle WA, US
Cathleen Zimmerman - Bainbridge Island WA, US
David Perry - Woodinville WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133, 356326

Abstract:

Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.

US Patent:

8103080, Jan 24, 2012

Filed:

Mar 16, 2005

Appl. No.:

10/593016

Inventors:

Thaddeus C George - Seattle WA, US
David A Basiji - Seattle WA, US
Brian E Hall - Seattle WA, US
William E Ortyn - Bainbridge Island WA, US
Michael J Seo - Mercer Island WA, US
Philip J Morrissey - Bellevue WA, US
Cathleen A Zimmerman - Bainbridge Island WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133, 382128, 382181, 382190, 382209, 382224, 356300, 356337, 356338, 435 4, 436164, 436171, 436172, 436 63, 702 19, 702 21

Abstract:

Provided are methods for determining and analyzing photometric and morphogenic features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.

US Patent:

8175371, May 8, 2012

Filed:

Jul 26, 2011

Appl. No.:

13/191270

Inventors:

Thaddeus C. George - Seattle WA, US
David A. Basiji - Seattle WA, US
Brian E. Hall - Seattle WA, US
William E. Ortyn - Bainbridge Island WA, US
Michael E. Seo - Mercer Island WA, US
Phillip J. Morrissey - Bellevue WA, US
Cathleen A. Zimmerman - Bainbridge Island WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133, 382128, 382181, 382190, 382209, 382224, 356300, 356337, 356338, 435 4, 436164, 436171, 436172, 436 63, 702 19, 702 21

Abstract:

Provided are methods for determining and analyzing photometric and morphogenic features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.

US Patent:

8406498, Mar 26, 2013

Filed:

Dec 4, 2009

Appl. No.:

12/631795

Inventors:

William Ortyn - Bainbridge Island WA, US
David Basiji - Seattle WA, US
Philip Morrissey - Bellevue WA, US
Thaddeus George - Seattle WA, US
Brian Hall - Seattle WA, US
Cathleen Zimmerman - Bainbridge Island WA, US
David Perry - Woodinville WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133

Abstract:

Multimodal or multispectral images of cells comprising a population of cells are simultaneously collected. Photometric and/or morphometric image features identifiable in the images are used to identify differences between first and second populations of cells. The differences can include changes in a relative percentage of different cell types in each population, or a change in a first type of cell present in the first population of cells and the same type of cell in the second population of cells. The changes may be indicative of a disease state, indicative of a relative effectiveness of a therapy, or indicative of a health of the person from whom the cells populations were obtained.

US Patent:

8571294, Oct 29, 2013

Filed:

Nov 22, 2011

Appl. No.:

13/302941

Inventors:

Thaddeus C. George - Seattle WA, US
David A. Basiji - Seattle WA, US
Brian E. Hall - Seattle WA, US
William E. Ortyn - Bainbridge Island WA, US
Michael E. Seo - Mercer Island WA, US
Phillip J. Morrissey - Bellevue WA, US
Cathleen A. Zimmerman - Bainbridge Island WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133, 382128, 382181, 382190, 382209, 382224, 356300, 356337, 356338, 435 4, 436164, 436171, 436172, 436 63, 702 19, 702 21

Abstract:

Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.

US Patent:

8571295, Oct 29, 2013

Filed:

Mar 13, 2013

Appl. No.:

13/802439

Inventors:

David A. Basiji - Seattle WA, US
Brian E. Hall - Seattle WA, US
William E. Ortyn - Bainbridge Island WA, US
Michael J. Seo - Westport CT, US
Philip J. Morrissey - Bellevue WA, US
Cathleen A. Zimmerman - Bainbridge Island WA, US

Assignee:

Amnis Corporation - Seattle WA

International Classification:

G06K 9/00

US Classification:

382133, 382128, 382181, 382190, 382209, 382224, 356300, 356337, 356338, 435 4, 436164, 436171, 436172, 436 63, 702 19, 702 21

Abstract:

Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.