Christian Elliott Gruber

US Patent:

6589737, Jul 8, 2003

Filed:

May 19, 2000

Appl. No.:

09/574111

Inventors:

Christian E. Gruber - Frederick MD
Po-Jen Shih - Columbia MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536 253

Abstract:

The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.

US Patent:

7037661, May 2, 2006

Filed:

Apr 3, 2003

Appl. No.:

10/405608

Inventors:

Christian E. Gruber - Frederick MD, US
Po-Jen Shih - Columbia MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/00

US Classification:

435 6, 435 911, 435 912, 536 253

Abstract:

The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.

US Patent:

7074556, Jul 11, 2006

Filed:

Feb 29, 2000

Appl. No.:

09/515513

Inventors:

Wu Bo Li - North Potomac MD, US
Joel A. Jessee - Mt. Airy MD, US
Christian E. Gruber - Frederick MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

435 6, 435 5, 435 15, 435 911, 435 912, 435 68, 435 70, 530350, 530351, 530324

Abstract:

The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.

US Patent:

20010036651, Nov 1, 2001

Filed:

Feb 5, 1998

Appl. No.:

09/018989

Inventors:

CHRISTIAN GRUBER - FREDERICK MD, US
JOEL A. JESSEE - MT. AIRY MD, US
JHY-JHU LIN - GAITHERSBURG MD, US

International Classification:

C12Q001/68
G01N033/53
C07H021/04
C12P019/34

US Classification:

435/091100, 435/006000, 435/007100, 435/007500, 435/091200, 536/024300, 536/024330

Abstract:

The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.

US Patent:

20020039744, Apr 4, 2002

Filed:

Dec 5, 2001

Appl. No.:

10/002158

Inventors:

Christian Gruber - Frederick MD, US
Joel Jessee - Mt. Airy MD, US
Jhy-Jhu Lin - Gaithersburg MD, US

International Classification:

C12Q001/68
G01N033/53
C07H021/04
C12N015/87

US Classification:

435/006000, 435/455000, 435/007100, 536/023100

Abstract:

The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.

US Patent:

20020064837, May 30, 2002

Filed:

May 7, 1999

Appl. No.:

09/306986

Inventors:

THUAN QUOC TRINH - GAITHERSBURG MD, US
CHRISTIAN ELLIOTT GRUBER - FREDERICK MD, US

International Classification:

C12Q001/68

US Classification:

435/091100, 435/006000, 435/091200

Abstract:

The subject invention provides novel compositions containing one or more peptides or polypeptides which possess ribonuclease activity. Preferably the ribonucleases for inclusion in the compositions are ribonucleases that substantially lack deoxyribonuclease activity. A preferred embodiment is a composition comprising RNase A or RNase T1. Another aspect of the invention is to provide methods for synthesizing nucleic acids, typically DNA, using the compositions of the invention or one or more ribonucleases. Another aspect of the invention involves the use of the subject method of nucleic acid synthesis to carry out the synthesis step in a polymerase chain reaction experiment. Yet another aspect of the invention is to provide kits for the synthesis of nucleic acids, wherein the kits comprise one or more peptides or polypeptides that possess ribonuclease activity.

US Patent:

20040185493, Sep 23, 2004

Filed:

Apr 5, 2004

Appl. No.:

10/816886

Inventors:

Christian Gruber - Frederick MD, US
Joel Jessee - Mount Airy MD, US

Assignee:

Invitrogen Corporation

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

The present invention is directed to methods for the production and isolation of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), which may be single- or double-stranded. Additionally, the invention concerns selection and isolation of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation.

US Patent:

20060127936, Jun 15, 2006

Filed:

Jan 12, 2006

Appl. No.:

11/330358

Inventors:

Christian Gruber - Frederick MD, US
Po-Jen Shih - Columbia MD, US

Assignee:

INVITROGEN CORPORATION - CARLSBAD CA

International Classification:

C12Q 1/68
C12N 9/22
C07H 21/04

US Classification:

435006000, 435199000, 536025320

Abstract:

The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics.