David M Diamond

US Patent:

6861234, Mar 1, 2005

Filed:

Apr 28, 2000

Appl. No.:

09/561074

Inventors:

John J. L. Simard - Northridge CA, US
David C. Diamond - Duarte CA, US

Assignee:

MannKind Corporation - Valencia CA

International Classification:

C12Q001/02

US Classification:

435 29, 435 4, 435 23, 435 78, 436 86, 436 89, 702 19

Abstract:

A method of epitope discovery comprising the step of selecting an epitope from a population of peptide fragments of an antigen associated with a target cell, wherein the fragments have a known or predicted affinity for a major histocompatibility complex class I receptor peptide binding cleft, wherein the epitope selected corresponds to a proteasome cleavage product of the target cell.

US Patent:

7232682, Jun 19, 2007

Filed:

Feb 10, 2004

Appl. No.:

10/777053

Inventors:

John J. L. Simard - Vancouver, CA
David C. Diamond - West Hills CA, US
Zhiyong Qiu - Los Angeles CA, US
Xiang-Dong Lei - West Hills CA, US

Assignee:

Mannkind Corporation - Valencia CA

International Classification:

C12N 15/12
A61K 39/00
A61K 38/00
A01N 43/00
C07K 14/435

US Classification:

4353201, 536 234, 536 235, 530350, 530403, 514 2, 514 44, 4241851, 4242771

Abstract:

The invention disclosed herein is directed to methods of identifying a polypeptide suitable for epitope liberation including, for example, the steps of identifying an epitope of interest; providing a substrate polypeptide sequence including the epitope, wherein the substrate polypeptide permits processing by a proteasome; contacting the substrate polypeptide with a composition including the proteasome, under conditions that support processing of the substrate polypeptide by the proteasome; and assaying for liberation of the epitope. The invention further relates to vectors including a housekeeping epitope expression cassette and also vectors including epitope cluster regions. The housekeeping epitope(s) can be derived from a target-associated antigen. The housekeeping epitope can be liberatable, that is capable of liberation, from a translation product of the cassette by immunoproteasome processing. The invention also relates to a method of activating a T cell comprising contacting a substrate polypeptide with an APC and contacting the APC with a T cell.

US Patent:

7252824, Aug 7, 2007

Filed:

Mar 7, 2002

Appl. No.:

10/094699

Inventors:

John J. L. Simard - Northridge CA, US
David C. Diamond - West Hills CA, US

Assignee:

Mannkind Corporation - Valencia CA

International Classification:

A61K 39/00

US Classification:

4241841, 424 91, 424 92, 435 691, 435 701, 435183, 530350, 530380, 530385, 5303871, 5303873

Abstract:

Disclosed herein are immunogenic compositions, methods of designing immunogenic compositions, methods of treatment using immunogenic compositions, methods of evaluating cell-mediated immunity resulting from immunogenic compositions, research models, and methods of making research models, all of which relate to targeting tumor vasculature.

US Patent:

8084592, Dec 27, 2011

Filed:

Jun 16, 2006

Appl. No.:

11/455279

Inventors:

Adrian Ion Bot - Valencia CA, US
Chih-Sheng Chiang - Chatsworth CA, US
David C. Diamond - West Hills CA, US
Jian Gong - Northridge CA, US
Kent Andrew Smith - Ventura CA, US
Liping Liu - Manassas VA, US
Xiping Liu - Temple City CA, US
Zhiyong Qiu - Los Angeles CA, US

Assignee:

MannKind Corporation - Valencia CA

International Classification:

C07H 21/02

US Classification:

536 231, 530328, 514 44 R, 514 11

Abstract:

The present invention provides a method of treating a cell proliferative disease such as cancer by providing to a subject in need thereof an immunogenic composition comprising plasmid and peptide(s) or analogues thereof. In embodiments of the present invention there is provided methods and compositions for inducing, entraining, and/or amplifying the immune response to MHC class-I restricted epitopes of carcinoma antigens to generate an effective anti-cancer immune response.

US Patent:

8252916, Aug 28, 2012

Filed:

Apr 30, 2004

Appl. No.:

10/837217

Inventors:

John J. L. Simard - Vancouver, CA
David C. Diamond - West Hills CA, US
Zhiyong Qiu - Los Angeles CA, US
Xiang-Dong Lei - West Hills CA, US

Assignee:

MannKind Corporation - Valencia CA

International Classification:

C07H 21/04
C07K 1/00

US Classification:

536 235, 536 242

Abstract:

The invention disclosed herein is directed to methods of identifying a polypeptide suitable for epitope liberation including, for example, the steps of identifying an epitope of interest; providing a substrate polypeptide sequence including the epitope, wherein the substrate polypeptide permits processing by a proteasome; contacting the substrate polypeptide with a composition including the proteasome, under conditions that support processing of the substrate polypeptide by the proteasome; and assaying for liberation of the epitope. The invention further relates to vectors including a housekeeping epitope expression cassette. The invention relates to epitope cluster regions and to vectors including epitope cluster regions. The invention also relates to a method of activating a T cell comprising contacting a substrate polypeptide with an APC and contacting the APC with a T cell.

US Patent:

8637305, Jan 28, 2014

Filed:

Nov 7, 2002

Appl. No.:

10/292413

Inventors:

John J. L. Simard - Vancouver, CA
David C. Diamond - West Hills CA, US
Zhiyong Qiu - Los Angeles CA, US
Xiang-Dong Lei - West Hills CA, US

Assignee:

Mannkind Corporation - Sylmar CA

International Classification:

C12N 15/63
A61K 38/04
C07K 4/00

US Classification:

4353201

Abstract:

The invention disclosed herein is directed to methods of identifying a polypeptide suitable for epitope liberation including, for example, the steps of identifying an epitope of interest; providing a substrate polypeptide sequence including the epitope, wherein the substrate polypeptide permits processing by a proteasome; contacting the substrate polypeptide with a composition including the proteasome, under conditions that support processing of the substrate polypeptide by the proteasome; and assaying for liberation of the epitope. The invention further relates to vectors including a housekeeping epitope expression cassette. The housekeeping epitope(s) can be derived from a target-associated antigen, and the housekeeping epitope can be liberatable, that is capable of liberation, from a translation product of the cassette by immunoproteasome processing. The invention also relates to a method of activating a T cell comprising contacting a substrate polypeptide with an APC and contacting the APC with a T cell.

US Patent:

20030138808, Jul 24, 2003

Filed:

Aug 20, 2002

Appl. No.:

10/225568

Inventors:

John Simard - Vancouver, CA
Xiang-Dong Lei - West Hills CA, US
David Diamond - West Hills CA, US

International Classification:

C12Q001/68
C07H021/04

US Classification:

435/006000, 536/023200

Abstract:

The invention provides a nucleic acid construct encoding a housekeeping epitope derived from an antigen associated with a target cell. The construct can further include a second sequence encoding a housekeeping epitope or an immune epitope. The construct can be used as a vaccine against numerous kinds of target cells, including neoplastic cells and cells infected with an intracellular virus.

US Patent:

20030215425, Nov 20, 2003

Filed:

Dec 7, 2001

Appl. No.:

10/026066

Inventors:

John Simard - Northridge CA, US
David Diamond - West Hills CA, US

International Classification:

A61K045/00
A61K038/19
A61K038/20
A61K048/00
C07H021/02
C07H021/04
C12N005/08

US Classification:

424/093700, 435/372000, 424/085100, 424/085200, 514/044000, 536/023100

Abstract:

Disclosed herein are vaccines and methods for inducing an immune response against cancer cells and cells infected with intracellular parasites. Vaccines having housekeeping epitopes are disclosed. The housekeeping epitope is formed by housekeeping proteasomes in peripheral cells, but not by professional antigen presenting cells. A vaccine containing a housekeeping epitope that is derived from an antigen associated with a peripheral target cell can thus direct an immune response against the target cell. Methods of treatment are also disclosed, which involve administering a vaccine having a housekeeping epitope.