David A Holman

US Patent:

7090774, Aug 15, 2006

Filed:

Oct 23, 1998

Appl. No.:

09/177902

Inventors:

David A. Holman - Richland WA, US
Cynthia J. Bruckner-Lea - Richland WA, US
Fred J. Brockman - Kennewick WA, US
Darrell P. Chandler - Richland WA, US

Assignee:

Battelle Memorial Institute - Richland WA

International Classification:

B01D 15/08

US Classification:

210656, 210657, 210670, 210675, 210691

Abstract:

The invention encompasses a method of packing and unpacking a column chamber. A mixture of a fluid and a matrix material are introduced through a column chamber inlet so that the matrix material is packed within a column chamber to form a packed column. The column chamber having the column chamber inlet or first port for receiving the mixture further has an outlet port and an actuator port. The outlet port is partially closed for capturing the matrix material and permitting the fluid to flow therepast by rotating relative one to the other of a rod placed in the actuator port. Further rotation relative one to the other of the rod and the column chamber opens the outlet and permits the matrix material and the fluid to flow therethrough thereby unpacking the matrix material from the column chamber.

US Patent:

7892856, Feb 22, 2011

Filed:

Dec 26, 2006

Appl. No.:

11/646216

Inventors:

Jay W. Grate - West Richland WA, US
Cynthia J. Bruckner-Lea - Richland WA, US
David A. Holman - Las Vegas NV, US

Assignee:

Battelle Memorial Institute - Richland WA

International Classification:

G01N 33/53

US Classification:

436526, 436 43, 436 45, 436 46, 422 31

Abstract:

Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection/resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.

US Patent:

20030095897, May 22, 2003

Filed:

Aug 31, 2001

Appl. No.:

09/944816

Inventors:

Jay Grate - West Richland WA, US
Cynthia Bruckner-Lea - Richland WA, US
David Holman - Las Vegas NV, US

International Classification:

B01J019/08

US Classification:

422/186000

Abstract:

Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection/resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.

US Patent:

20030164335, Sep 4, 2003

Filed:

Apr 24, 2002

Appl. No.:

10/132498

Inventors:

Jay Grate - West Richland WA, US
Cindy Bruckner-Lea - Richland WA, US
Darrell Chandler - West Richland WA, US
David Holman - Las Vegas NV, US

International Classification:

B01D015/00

US Classification:

210/660000

Abstract:

A method for manipulating small particles in a microfluidic system wherein a fluid flow through a tolerance of the microfluidic system is used to capture large particles, which are then used, in turn, to capture small particles.

US Patent:

20060096903, May 11, 2006

Filed:

Dec 5, 2005

Appl. No.:

11/294713

Inventors:

David Holman - Richland WA, US
Cynthia Bruckner-Lea - Richland WA, US
Fred Brockman - Kennewick WA, US
Darrell Chandler - Richland WA, US

International Classification:

B01D 15/00

US Classification:

210198200, 210263000

Abstract:

In one aspect, the invention encompasses a method of packing and unpacking a column chamber. A mixture of a fluid and a matrix material are introduced through a column chamber inlet so that the matrix material is packed within a column chamber to form a packed column. After the packing, the matrix material is unpacked from the column chamber without moving the column chamber. More specifically, the column chamber having the column chamber inlet or first port for receiving the mixture further has an outlet port and an actuator port. The outlet port is partially closed for capturing the matrix material and permitting the fluid to flow therepast by rotating relative one to the other of a rod placed in the actuator port. Further rotation relative one to the other of the rod and the column chamber opens the outlet and permits the matrix material and the fluid to flow therethrough thereby unpacking the matrix material from the column chamber. In another aspect, the invention encompasses a method of purifying a component of a sample. A column chamber having an inlet end, an outlet end and an actuator end is provided. Flow of matrix material is obstructed by a rod with a binary end inserted in the actuator end. A suspension of the first fluid and the matrix material is flowed into the column chamber to form a packed column of the matrix material within the column chamber. The matrix material is configured to selectively retain a component of the sample. The sample is flowed through the packed column and past the rod to separate the component from the rest of the sample. The rod or the column chamber is rotated with respect to the other to open the outlet end and to remove the matrix material from the column chamber. In yet another aspect, the invention encompasses a system for column-based separations. The system comprises a fluid passageway containing a column chamber and a flow path in fluid communication with the column chamber. The flow path is partially obstructed by a rod. The flow path extends through the column chamber and through the outlet end. The flow path is configured to form a packed column within the column chamber when a suspension of the fluid and the column matrix material is flowed along the flow path.

US Patent:

61593788, Dec 12, 2000

Filed:

Feb 23, 1999

Appl. No.:

9/255758

Inventors:

David A. Holman - Richland WA
Jay W. Grate - West Richland WA
Cynthia J. Bruckner-Lea - Richland WA

Assignee:

Battelle Memorial Institute - Richland WA

International Classification:

B01D 3506

US Classification:

210695

Abstract:

The present invention is an apparatus and method for handling magnetic particles suspended in a fluid, relying upon the known features of a magnetic flux conductor that is permeable thereby permitting the magnetic particles and fluid to flow therethrough; and a controllable magnetic field for the handling. The present invention is an improvement wherein the magnetic flux conductor is a monolithic porous foam.

Address:

609 Bremerton Pl NE, Renton, WA 98059

VIN:

4T1FA38P37U121405

Make:

TOYOTA

Model:

CAMRY SOLARA

Year:

2007