David M Kaplan

US Patent:

20110076384, Mar 31, 2011

Filed:

Apr 8, 2009

Appl. No.:

12/934666

Inventors:

Christopher Cannizzaro - Washington DC, US
Michael L. Lovett - Peabody MA, US
David L. Kaplan - Concord MA, US

Assignee:

TRUSTEES OF TUFTS COLLEGE - Medford MA
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK - New York NY

International Classification:

B05C 11/00
A61L 33/00

US Classification:

427 21, 118606

Abstract:

A system and method for making a biomaterial device includes a support structure providing a shape for a biomaterial device. At least one applicator has a supply of biomaterial solution and is positioned along the support structure. The at least one applicator forms a biomaterial fiber by applying shear force to the biomaterial solution and delivering the biomaterial fiber to the support structure. A controller causes relative movement between the support structure and the at least one applicator, and the biomaterial fiber is arranged on the support structure according to the relative movement to form the biomaterial device. The biomaterial may be silk fibroin which may be wound onto a reciprocating and rotating mandrel. Control over the properties of the biomaterial device is achieved through appropriate selection of material processing, winding strategy, and post-winding processing.

US Patent:

20130004930, Jan 3, 2013

Filed:

Jul 2, 2012

Appl. No.:

13/540359

Inventors:

Peter Floyd Sorenson - New Haven CT, US
David Michael Kaplan - Cromwell CT, US

International Classification:

G09B 5/00

US Classification:

434350

Abstract:

The Learner Interaction Monitoring Systems (LiMS) is a web-based application that can interface with any web-based course delivery platform to transform the online learning environment into an active observer of learner engagement. The LiMS ‘event capture model’ collects detailed real-time data on learner behavior in self-directed online learning environments, and interprets these data by drawing on behavioral research. The LiMS offers education and training managers in corporate contexts a valuable tool for the evaluation of learner performance and course design. By allowing more detailed demonstration of ROI in education and training, LiMS allows managers to make the case for web based courseware that reflects appropriate and evidence-based instructional design, rather than budgetary constraints.

US Patent:

52945413, Mar 15, 1994

Filed:

Jul 13, 1992

Appl. No.:

7/912590

Inventors:

David S. Kaplan - Fairfax VA
Grace L. Picciolo - Fort Washington MD
Edward P. Mueller - Bayridge MD

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12Q 102
C12Q 106

US Classification:

435 29

Abstract:

A chemiluminescence method for continuously monitoring in real time the generation of oxidative products such as hydrogen peroxide and superoxide from in vitro cell-biomaterial interactions using cell lines such as Human Leukemic cells (HL-60); tumor cell line hybridomas; cells lacking the respiratory burst such as Chronic Granulomatous Disease cells as controls; Monocytic cell lines; Primary Human cells such as monocytes, pmns, fibroblasts, endothelial cells; and whole and isolated blood cells. The oxidative products have the potential for the degradation of the biomaterial and thus this information can be used to aid in predicting the functional lifetime of the biomaterial when it is used to fabricate an implanted medical device. Further, this method may be used to determine the amount of activation of biological cells which is inferred from the amount of oxidative products. This activation level aids in the determination of the degree of the inflammatory response and thus influences the degree of acceptance of the biomaterial.

US Patent:

47771330, Oct 11, 1988

Filed:

Nov 26, 1985

Appl. No.:

6/801965

Inventors:

Grace L. Picciolo - Rockville MD
David S. Kaplan - Fairfax VA

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12Q 102
G01N 33571
G01N 3500
G01N 33552

US Classification:

435 29

Abstract:

The present invention discloses a device and a process for quantitation of Toxoplasma gondii and Treponema pallidum antibody titer in a biological sample by immunofluorescent photometric microscopy. The process comprises: (a) reacting a specimen of said sample with an immunofluorescent reagent in a mounting medium containing a protective agent in an amount sufficient to reduce fading of a fluorescent reaction product less than 25% of initial fluorescent intensity; (b) localizing the specimen under transmitted, visible light; (c) reducing the effect of counterstain intensity by filters in emission light path; (d) measuring the sample using a fast shutter; (e) calibrating the photometer used in said microscopy by a stable fluorophore; (f) recording intensity of fluorescence of said specimen compared to standard negative and positive controls; (g) reducing effect of polar staining by substracting the corrected intensity of corresponding dilution of the negative control from the sample reading; and (h) assigning a numerical endpoint for serum antibody levels against Toxoplasma gondii or Treponema pallidum.

US Patent:

46222910, Nov 11, 1986

Filed:

Jun 11, 1984

Appl. No.:

6/619325

Inventors:

Grace L. Picciolo - Fort Washington MD
David S. Kaplan - Fairfax VA

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

C12Q 100
C12Q 102
G01N 33552
G01N 33542

US Classification:

435 4

Abstract:

The present invention discloses a device and a process for quantitation of end point in the formation of fluorescent reaction product in microfluorophotometry. The process comprises: (a) incorporating a protective agent in a suitable mounting medium in an amount sufficient to reduce fading of fluorescent reaction product less than 25% of initial fluorescent intensity; (b) calibrating photometer used in said microscopy with a stable emitter; and (c) recording the intensity of fluorescence of said fluorescent reaction product by means for measuring light intensity. The invention also includes a device for calibration of the photometer and a kit comprising separate containers for suitable mounting medium, buffer, suitable immunofluorescent reagents, fading retardant means, a photometer calibrating device and the like and optional instructions.

US Patent:

20230051780, Feb 16, 2023

Filed:

Jun 17, 2022

Appl. No.:

17/843752

Inventors:

Surjeet Rajendran - Ellicott City MD, US
David Elazzar Kaplan - Baltimore MD, US

International Classification:

G06N 10/20

Abstract:

Protocols to exploit non-linear quantum mechanical effects to parallelize any process (such as computational, mechanical or biological), inhibit the dilution of non-linear quantum mechanical effects and enhance the shot noise limit of quantum measurement devices are described. The key concept behind these protocols is the use of a device that can communicate across different arms of a quantum superposition even in the presence of decoherence using non-linear quantum mechanical interactions (such as the expectation values of classical sources of electromagnetism and gravitation). By communicating across different arms of a quantum superposition, the protocol can collect the results of parallelizable tasks performed in each arm of the superposition, arrive at well defined final states across the superposition and use the strength of the observed non-linearity to measure the properties of the superposition itself.