David L Liu

US Patent:

6360457, Mar 26, 2002

Filed:

Jun 28, 2000

Appl. No.:

09/607516

Inventors:

Yi-Ming Qui - City of Industry CA 91748
David Liu - City of Industry CA 91748
Joanne Yang - City of Industry CA 91748

International Classification:

A61F 514

US Classification:

36140, 36136, 36141, 36 43, 600 15

Abstract:

A health care shoe, which can continuously provide adequate magnetic field arranged to penetrate through the wearers foot for improving the wearers blood circulation and metabolism, includes a sole; an insole placed on top the sole, which has a plurality of magnet cavities formed and distributed thereon; a shoe body rigidly attached on top of the sole to define a foot chamber between the shoe body and the insole; a plurality of base magnet elements fittingly received in the magnet cavities respectively provided on the insole; and a plurality of upper magnet elements embedded inside the shoe body with respect to the positions of the base magnet elements located on the insole, wherein each of the upper magnet elements is arranged to constitute a transversal magnetic field penetrating through the foot chamber and flowing towards one of the base magnet elements. Moreover, the sole further has at least a medicine receiving cavity to receive a medicine unit which contains a medicine tablet made of painkiller or Chinese medical herb being incased therein. The medicine unit further includes a bottom magnet element positioned thereunder so as to produce magnetic filed around the medicine unit.

US Patent:

7045337, May 16, 2006

Filed:

Apr 19, 2002

Appl. No.:

10/126927

Inventors:

Peter Schultz - La Jolla CA, US
Lei Wang - San Diego CA, US
John Christopher Anderson - San Diego CA, US
Jason William Chin - San Diego CA, US
David R. Liu - Lexington MA, US
Thomas J. Magliery - North Haven CT, US
Eric L. Meggers - Philadelphia PA, US
Ryan A. Mehl - San Diego CA, US
Miro Pastrnak - San Diego CA, US
Steven William Santoro - San Diego CA, US
Zhiwen Zhang - San Diego CA, US

Assignee:

The Scripps Research Institute - La Jolla CA
The Regents of the University of California - Oakland CA

International Classification:

C12N 9/10
C12N 15/12
C12N 15/54
C12P 21/00

US Classification:

4352523, 435193, 435 914, 43525233, 435320, 435 15, 435 691, 536 232

Abstract:

The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

US Patent:

7070928, Jul 4, 2006

Filed:

Mar 19, 2002

Appl. No.:

10/101030

Inventors:

David R. Liu - Lexington MA, US
Zev Gartner - Somerville MA, US
Matthew W. Kanan - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

US Patent:

7083970, Aug 1, 2006

Filed:

Apr 19, 2002

Appl. No.:

10/126931

Inventors:

Peter Schultz - La Jolla CA, US
Lei Wang - San Diego CA, US
John Christopher Anderson - San Diego CA, US
Jason Chin - San Diego CA, US
David R. Liu - Lexington MA, US
Thomas J. Magliery - North Haven CT, US
Eric L. Meggers - La Jolla CA, US
Ryan Aaron Mehl - San Diego CA, US
Miro Pastrnak - San Diego CA, US
Stephen William Santoro - San Diego CA, US
Zhiwen Zhang - San Diego CA, US

Assignee:

The Scripps Research Institute - La Jolla CA
The Regents of the University of California - Oakland CA

International Classification:

C12N 9/10
C12N 15/12
C12N 15/54
C12P 21/00

US Classification:

4352523, 435193, 435 914, 43525233, 4353201, 435 15, 435 691

Abstract:

This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

US Patent:

7192739, Mar 20, 2007

Filed:

Mar 30, 2005

Appl. No.:

11/093808

Inventors:

David R. Liu - Lexington MA, US
Allen R. Buskirk - Provo UT, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12P 21/06
C12P 21/04

US Classification:

435 691, 435 697

Abstract:

Ligand-dependent inteins allow for modulation of a protein's activity in vivo. Upon binding of the ligand to the ligand-dependent intein inserted into the protein of interest, the hybrid protein undergoes protein splicing removing the intein. The activity of the spliced protein is then restored. A 4-hydroxytamoxifen-dependent intein based on the RecA intein is prepared and demonstrated in a variety of exteins contexts. The invention provides a system for engineering other ligand-dependent inteins and using them, including the ligand-dependent inteins themselves, hybrid proteins with the inserted ligand-dependent inteins, polynucleotides encoding inteins and hybrid proteins, and engineered cells. Kits with the materials and reagents necessary for preparing and using ligand-dependent inteins are also included.

US Patent:

7223545, May 29, 2007

Filed:

Sep 24, 2004

Appl. No.:

10/949163

Inventors:

David R. Liu - Lexington MA, US
Zev J. Gartner - Somerville MA, US
Matthew W. Kanan - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07K 1/00

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 530350

Abstract:

Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

US Patent:

7354761, Apr 8, 2008

Filed:

Oct 18, 2005

Appl. No.:

11/254170

Inventors:

Peter G. Schultz - La Jolla CA, US
Lei Wang - San Diego CA, US
John Christopher Anderson - San Diego CA, US
Jason Chin - Cambridge, GB
David R. Liu - Lexington MA, US
Thomas J. Magliery - North Haven CT, US
Eric L. Meggers - Philadelphia PA, US
Ryan Aaron Mehl - Lancaster PA, US
Miro Pastrnak - San Diego CA, US
Steven William Santoro - Cambridge MA, US
Zhiwen Zhang - San Diego CA, US

Assignee:

The Scripps Research Institute - La Jolla CA
The Regents of the University of California - Oakland CA

International Classification:

C12N 9/10
C12N 15/12
C12N 15/54
C12N 21/00

US Classification:

435325, 435193, 435 914, 4352523, 43525233, 435320, 435 691, 43525411, 4352542, 536 232

Abstract:

This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

US Patent:

7368275, May 6, 2008

Filed:

Oct 18, 2005

Appl. No.:

11/254161

Inventors:

Peter Schultz - La Jolla CA, US
Lei Wang - San Diego CA, US
John Christopher Anderson - San Diego CA, US
Jason W. Chin - Cambridge, GB
David R. Liu - Lexington MA, US
Thomas J. Magliery - North Haven CT, US
Eric Meggers - Philadelphia PA, US
Ryan Aaron Mehl - Lancaster PA, US
Miro Pastrnak - San Diego CA, US
Steven William Santoro - Cambridge MA, US
Zhiwen Zhang - San Diego CA, US

Assignee:

The Scripps Research Institute - La Jolla CA
The Regents of the University of California - Oakland CA

International Classification:

C12N 9/10
C12N 15/12
C12N 15/54
C12P 21/00

US Classification:

4352523, 435193, 435 914, 43525233, 435320, 435 15, 435 691, 536 232

Abstract:

The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.