David S Liu

US Patent:

6360457, Mar 26, 2002

Filed:

Jun 28, 2000

Appl. No.:

09/607516

Inventors:

Yi-Ming Qui - City of Industry CA 91748
David Liu - City of Industry CA 91748
Joanne Yang - City of Industry CA 91748

International Classification:

A61F 514

US Classification:

36140, 36136, 36141, 36 43, 600 15

Abstract:

A health care shoe, which can continuously provide adequate magnetic field arranged to penetrate through the wearers foot for improving the wearers blood circulation and metabolism, includes a sole; an insole placed on top the sole, which has a plurality of magnet cavities formed and distributed thereon; a shoe body rigidly attached on top of the sole to define a foot chamber between the shoe body and the insole; a plurality of base magnet elements fittingly received in the magnet cavities respectively provided on the insole; and a plurality of upper magnet elements embedded inside the shoe body with respect to the positions of the base magnet elements located on the insole, wherein each of the upper magnet elements is arranged to constitute a transversal magnetic field penetrating through the foot chamber and flowing towards one of the base magnet elements. Moreover, the sole further has at least a medicine receiving cavity to receive a medicine unit which contains a medicine tablet made of painkiller or Chinese medical herb being incased therein. The medicine unit further includes a bottom magnet element positioned thereunder so as to produce magnetic filed around the medicine unit.

US Patent:

6461129, Oct 8, 2002

Filed:

Feb 23, 2001

Appl. No.:

09/791515

Inventors:

David Liu - Vernon Hills IL

Assignee:

MAT Automotive Inc. - Buffalo Grove IL

International Classification:

F03C 200

US Classification:

418 552, 418 555, 418 57, 418107

Abstract:

A scroll type apparatus for fluid displacement is disclosed. In one embodiment, the apparatus includes an adjustment mechanism capable of being adjusted after assembly of the apparatus to close an axial gap between scroll members and account for manufacturing tolerances in apparatus components. In another embodiment, the apparatus includes an orbital scroll of two portions, with a supporting portion surrounding an eccentric bearing of higher density than that of a scroll portion. The center of mass of the orbital scroll is thus moved towards the eccentric bearing to reduce torquing of the scroll as it orbits. In a further embodiment, the apparatus includes an orbital scroll having two portions, a supporting portion surrounding an eccentric bearing having a lower coefficient of thermal expansion than that of a scroll portion, to reduce thermal expansion of the supporting portion, reducing misaligmnent of the eccentric orbital scroll on the bearing.

US Patent:

7070928, Jul 4, 2006

Filed:

Mar 19, 2002

Appl. No.:

10/101030

Inventors:

David R. Liu - Lexington MA, US
Zev Gartner - Somerville MA, US
Matthew W. Kanan - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

US Patent:

7192739, Mar 20, 2007

Filed:

Mar 30, 2005

Appl. No.:

11/093808

Inventors:

David R. Liu - Lexington MA, US
Allen R. Buskirk - Provo UT, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12P 21/06
C12P 21/04

US Classification:

435 691, 435 697

Abstract:

Ligand-dependent inteins allow for modulation of a protein's activity in vivo. Upon binding of the ligand to the ligand-dependent intein inserted into the protein of interest, the hybrid protein undergoes protein splicing removing the intein. The activity of the spliced protein is then restored. A 4-hydroxytamoxifen-dependent intein based on the RecA intein is prepared and demonstrated in a variety of exteins contexts. The invention provides a system for engineering other ligand-dependent inteins and using them, including the ligand-dependent inteins themselves, hybrid proteins with the inserted ligand-dependent inteins, polynucleotides encoding inteins and hybrid proteins, and engineered cells. Kits with the materials and reagents necessary for preparing and using ligand-dependent inteins are also included.

US Patent:

7223545, May 29, 2007

Filed:

Sep 24, 2004

Appl. No.:

10/949163

Inventors:

David R. Liu - Lexington MA, US
Zev J. Gartner - Somerville MA, US
Matthew W. Kanan - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07K 1/00

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243, 536 2431, 536 2432, 536 2433, 530350

Abstract:

Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

US Patent:

7442160, Oct 28, 2008

Filed:

May 31, 2005

Appl. No.:

11/141542

Inventors:

David R. Liu - Lexington MA, US
Zev J. Gartner - Somerville MA, US
Matthew W. Kanan - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C40B 30/04
C40B 10/00
C40B 30/08
C40B 40/00
C40B 50/10
C40B 70/00
C12Q 1/68
C07H 21/02

US Classification:

506 9, 506 1, 506 11, 506 13, 506 28, 506 41, 435 6, 435 912, 536 231

Abstract:

Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

US Patent:

7476500, Jan 13, 2009

Filed:

Mar 19, 2002

Appl. No.:

10/102056

Inventors:

David R. Liu - Lexington MA, US
Zev Gartner - Somerville MA, US
Daniel M. Rosenbaum - Cambridge MA, US
Mathias Gruen - Jena, DE
Jeffrey Doyon - Allston MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q 1/68
C12N 9/22

US Classification:

435 6, 435199

Abstract:

The present invention provides in vivo systems in which activity of a biological cleavage enzyme, such as a site-specific recombinase, a homing endonuclease, or an intein, is linked to cell viability and therefore can be selected. The invention further provides methods of making cells in which the activity of a biological cleavage enzyme is linked to viability, as well as methods of identifying new biological cleavage enzymes, including enzymes having altered site specificity, using such cells.

US Patent:

7476734, Jan 13, 2009

Filed:

Dec 6, 2005

Appl. No.:

11/295155

Inventors:

David R. Liu - Lexington MA, US

Assignee:

Helicos Biosciences Corporation - Cambridge MA

International Classification:

C07H 19/04
C07H 19/20

US Classification:

536 2621, 536 221, 536 253, 536 2532, 536 261, 536 262

Abstract:

The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.

Address:

4564 S Christiana Ave, Chicago, IL 60632

Phone:

7732540131

VIN:

2T2BK1BA4AC021564

Make:

LEXUS

Model:

RX 350

Year:

2010